BDP1/YNL039W Summary Help

Standard Name BDP1 1
Systematic Name YNL039W
Alias TFC5 2 , TFC7 3
Feature Type ORF, Verified
Description Essential subunit of RNA polymerase III transcription factor (TFIIIB); TFIIIB is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs (1 and see Summary Paragraph)
Name Description B Double Prime 4
Gene Product Alias B" 4 , TFIIIB90 4
Chromosomal Location
ChrXIV:555048 to 556832 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BDP1 GO evidence and references
  View Computational GO annotations for BDP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 62 genes
Resources
Classical genetics
conditional
null
unspecified
Large-scale survey
null
reduction of function
Resources
38 total interaction(s) for 21 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Far Western: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 8
  • Negative Genetic: 4
  • Phenotypic Enhancement: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 594
Molecular Weight (Da) 67,687
Isoelectric Point (pI) 6.93
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:555048 to 556832 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1785 555048..556832 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004984
SUMMARY PARAGRAPH for BDP1

The TFIIIB complex is a initiation factor for RNA polymerase III, which transcribes tRNAs, most small nuclear RNAs, and 5S rRNA (reviewed in 5). TFIIIB, comprised of TBP (Spt15p), Brf1p, and Bdp1p, directs pol III to the transcriptional start site of these genes and is itself recruited to a location immediately upstream of the transcriptional start site by the TFIIIC complex (6). TFIIIB binds to TFIIIC mainly through contact between TFIIIB subunits Brf1p and Bdp1p and the TFIIIC subunit Tfc4p, and between TBP and the TFIIIC subunit Tfc8p (7, 8). The TFIIIB footprint on promoter DNA is influenced by the DNA-binding site preferences of the TBP subunit and by the non-histone chromatin proteins Nhp6Ap and Nhp6bp (9 and reviewed in 10). TFIIIB function is also important for promoter opening (via the Brf1p and Bdp1p subunits), reinitiation of pol III transcription, and targeting and efficiency of Ty3 retrotransposition (11, 12, and 13). TFIIIB is also the target of Maf1-mediated repression of pol III transcription (reviewed in 14).

Last updated: 2010-02-26 Contact SGD

References cited on this page View Complete Literature Guide for BDP1
1) Ishiguro A, et al.  (2002) Essential roles of Bdp1, a subunit of RNA polymerase III initiation factor TFIIIB, in transcription and tRNA processing. Mol Cell Biol 22(10):3264-75
2) Kassavetis GA, et al.  (1995) Cloning, expression, and function of TFC5, the gene encoding the B" component of the Saccharomyces cerevisiae RNA polymerase III transcription factor TFIIIB. Proc Natl Acad Sci U S A 92(21):9786-90
3) Ruth J, et al.  (1996) A suppressor of mutations in the class III transcription system encodes a component of yeast TFIIIB. EMBO J 15(8):1941-9
4) Kassavetis GA, et al.  (2006) Mapping the principal interaction site of the Brf1 and Bdp1 subunits of Saccharomyces cerevisiae TFIIIB. J Biol Chem 281(20):14321-9
5) Geiduschek EP and Kassavetis GA  (2001) The RNA polymerase III transcription apparatus. J Mol Biol 310(1):1-26
6) Kassavetis GA, et al.  (1997) Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoter. Mol Cell Biol 17(9):5299-306
7) Chaussivert N, et al.  (1995) Complex interactions between yeast TFIIIB and TFIIIC. J Biol Chem 270(25):15353-8
8) Deprez E, et al.  (1999) A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein. Mol Cell Biol 19(12):8042-51
9) Kassavetis GA and Steiner DF  (2006) Nhp6 is a transcriptional initiation fidelity factor for RNA polymerase III transcription in vitro and in vivo. J Biol Chem 281(11):7445-51
10) Geiduschek EP  (2009) Without a license, or accidents waiting to happen. Annu Rev Biochem 78:1-28
11) Kassavetis GA, et al.  (2001) The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening. EMBO J 20(11):2823-34
12) Ferrari R, et al.  (2004) Distinct roles of transcription factors TFIIIB and TFIIIC in RNA polymerase III transcription reinitiation. Proc Natl Acad Sci U S A 101(37):13442-7
13) Yieh L, et al.  (2002) Mutational analysis of the transcription factor IIIB-DNA target of Ty3 retroelement integration. J Biol Chem 277(29):25920-8
14) Ciesla M and Boguta M  (2008) Regulation of RNA polymerase III transcription by Maf1 protein. Acta Biochim Pol 55(2):215-25