BDF1/YLR399C Summary Help

Standard Name BDF1 1
Systematic Name YLR399C
Feature Type ORF, Verified
Description Protein involved in transcription initiation; functions at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p; BDF1 has a paralog, BDF2, that arose from the whole genome duplication (2, 3 and see Summary Paragraph)
Name Description BromoDomain Factor 1, 2
Chromosomal Location
ChrXII:921596 to 919536 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All BDF1 GO evidence and references
  View Computational GO annotations for BDF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
Large-scale survey
246 total interaction(s) for 178 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 30
  • Biochemical Activity: 1
  • Co-localization: 1
  • Co-purification: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 72

Genetic Interactions
  • Dosage Rescue: 6
  • Negative Genetic: 22
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 6
  • Positive Genetic: 3
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 18
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 686
Molecular Weight (Da) 76,977
Isoelectric Point (pI) 5.86
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:921596 to 919536 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
This feature contains embedded feature(s): YLR399W-A
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2061 921596..919536 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004391

BDF1 was identified in a screen for mutants that affect transcription of a reporter snRNA (1). BDF1 mutants show a reduced rate of vegetative growth, sensitivity to DNA damaging agents, and are unable to undergo the meiotic divisions required for sporulation (4). Bdf1p is found in the nucleus throughout the cell cycle, and is localized uniformly along the length of chromosomes (4). This localization suggests that Bdf1p may be a chromatin component (4). Bdf1p has some regions of similarity to proteins from Drosophila and human (1); Bdf1p also contains two "bromodomains," regions that may be involved in interactions with histones (1).

Last updated: 2000-05-01 Contact SGD

References cited on this page View Complete Literature Guide for BDF1
1) Lygerou Z, et al.  (1994) The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs. Nucleic Acids Res 22(24):5332-40
2) Matangkasombut O, et al.  (2000) Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev 14(8):951-62
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Chua P and Roeder GS  (1995) Bdf1, a yeast chromosomal protein required for sporulation. Mol Cell Biol 15(7):3685-96