BAS1/YKR099W Summary Help

BAS1 BASIC INFORMATION

Standard Name BAS1
Systematic Name YKR099W
Feature Type ORF, Verified
Description Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes (1, 2, 3 and see Summary Paragraph)
Name Description BASal 4
GO Annotations All BAS1 GO evidence and references
    View Computational GO annotations for BAS1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs BAS1 Transcription Factor Binding Sites and References
  • TGACTC
Regulatory modules predicted: stressResponse ( 373 )
Mutant Phenotype All BAS1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions BAS1 All interactions details and references
38 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-Western: 2
  • Protein-RNA: 1
  • Two-hybrid: 11
Genetic Interactions
  • Phenotypic Enhancement: 16
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1
Sequence Information
ChrXI:635489 to 637924 | ORF Map | GBrowse
Gbrowse
Genetic position: 82 cM
Last Update Coordinates: 2005-12-15 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2436 635489..637924 2005-12-15 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001807

BAS1 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BAS1

SUMMARY PARAGRAPH for BAS1

BAS1 encodes a transcription factor involved in the expression of genes encoding enzymes acting in the histidine, purine, and pyrimidine biosynthetic pathways (5, 6, 4, 7, 4, 2). A genome-wide survey of genes regulated by Bas1p was performed by Denis et al. (5). Bas1p acts in conjunction with Grf10p (4) in a TATA-independent fashion (8). Bas1p has been shown to bind to a site containing a TGACTC hexanucleotide found in the ADE2 and ADE5,7 promoters (9). This motif is required for the regulation of ADE2 expression by Bas1p/Bas2p (2). A model for the action of Bas1p and Bas2p is that formation of a complex between the two proteins unmasks an activator function in Bas1p. Moreover, in adenine-replete cells, there may be a negative regulator that inhibits formation of this complex (10, 11). However, there is evidence that there may be other partners that can bind Bas1p (6). The N-terminal region of Bas1p is similar to those of proteins in the myb proto-oncogene family (1).

Last updated: 1999-07-01

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BAS1]

1) Tice-Baldwin K, et al.  (1989) BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science 246(4932):931-5
2) Daignan-Fornier B and Fink GR  (1992) Coregulation of purine and histidine biosynthesis by the transcriptional activators BAS1 and BAS2. Proc Natl Acad Sci U S A 89(15):6746-50
3) Mieczkowski PA, et al.  (2006) Global analysis of the relationship between the binding of the Bas1p transcription factor and meiosis-specific double-strand DNA breaks in Saccharomyces cerevisiae. Mol Cell Biol 26(3):1014-27
4) Arndt KT, et al.  (1987) Multiple global regulators control HIS4 transcription in yeast. Science 237(4817):874-80
5) Denis V, et al.  (1998) Role of the myb-like protein bas1p in Saccharomyces cerevisiae: a proteome analysis. Mol Microbiol 30(3):557-66
6) Denis V and Daignan-Fornier B  (1998) Synthesis of glutamine, glycine and 10-formyl tetrahydrofolate is coregulated with purine biosynthesis in Saccharomyces cerevisiae. Mol Gen Genet 259(3):246-55
7) Springer C, et al.  (1996) Amino acid and adenine cross-pathway regulation act through the same 5'-TGACTC-3' motif in the yeast HIS7 promoter. J Biol Chem 271(47):29637-43
8) Pellman D, et al.  (1990) TATA-dependent and TATA-independent transcription at the HIS4 gene of yeast. Nature 348(6296):82-5
9) Hovring I, et al.  (1994) DNA-binding domain and recognition sequence of the yeast BAS1 protein, a divergent member of the Myb family of transcription factors. J Biol Chem 269(26):17663-9
10) Zhang F, et al.  (1997) Evidence that complex formation by Bas1p and Bas2p (Pho2p) unmasks the activation function of Bas1p in an adenine-repressible step of ADE gene transcription. Mol Cell Biol 17(6):3272-83
11) Rolfes RJ, et al.  (1997) The transcriptional activators BAS1, BAS2, and ABF1 bind positive regulatory sites as the critical elements for adenine regulation of ADE5,7. J Biol Chem 272(20):13343-54