BAR1/YIL015W Summary

Standard Name	BAR1 1
Systematic Name	YIL015W
Alias	SST1 1 , 2
Feature Type	ORF, Verified
Description	Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest (2, 3, 4 and see Summary Paragraph)
Name Description	BARrier to the alpha factor response 1, 5

Chromosomal Location	ChrIX:322342 to 324105 \| ORF Map \| GBrowse

	Genetic position: -12 cM

Gene Ontology Annotations All BAR1 GO evidence and references

	View Computational GO annotations for BAR1
Molecular Function
Manually curated	aspartic-type endopeptidase activity (IDA, IMP)
Biological Process
Manually curated	adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion (IDA, IMP) peptide catabolic process (IDA)
Cellular Component
Manually curated	cell wall-bounded periplasmic space (TAS)

Mutant phenotypes All BAR1 Phenotype evidence and references

Classical genetics
null	pheromone sensitivity: increased
Large-scale survey
null	bud morphology: abnormal competitive fitness: decreased haploproficient hyperosmotic stress resistance: decreased toxin resistance: decreased transposable element transposition: increased vacuolar morphology: abnormal vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All BAR1 Interaction evidence and references

	15 total interaction(s) for 14 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 2
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 5 Phenotypic Enhancement: 3 Synthetic Growth Defect: 2 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All BAR1 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIX:322342 to 324105 | |

: -12 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1994-12-10

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1764	322342..324105	2011-02-03	1994-12-10

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000001277

SUMMARY PARAGRAPH for BAR1

Bar1p is a protease secreted into the periplasmic space of Mata cells that cleaves and inactivates alpha factor (2, 6). Mata cells that lack Bar1p are supersensitive to alpha factor-induced G1 arrest (2, 7). Transcription of BAR1 in alpha haploids and a/alpha diploids is repressed by the alpha2 protein and cofactors, and BAR1 transcription in Mata cells is stimulated by the presence of alpha factor (8, 9, 10). Bar1p is an aspartyl protease with similarity to pepsin, Pep4p, and Yap3p (11, 12, 6).

Last updated: 1999-12-31

References cited on this page View Complete Literature Guide for BAR1

1)	Sprague GF Jr and Herskowitz I (1981) Control of yeast cell type by the mating type locus. I. Identification and control of expression of the a-specific gene BAR1. J Mol Biol 153(2):305-21
2)	Chan RK and Otte CA (1982) Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol 2(1):21-9
3)	Ciejek E and Thorner J (1979) Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action. Cell 18(3):623-35
4)	Barkai N, et al. (1998) Protease helps yeast find mating partners. Nature 396(6710):422-3
5)	Hicks JB and Herskowitz I (1976) Evidence for a new diffusible element of mating pheromones in yeast. Nature 260(5548):246-8
6)	Ballensiefen W and Schmitt HD (1997) Periplasmic Bar1 protease of Saccharomyces cerevisiae is active before reaching its extracellular destination. Eur J Biochem 247(1):142-7
7)	Chan RK and Otte CA (1982) Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones. Mol Cell Biol 2(1):11-20
8)	Kronstad JW, et al. (1987) A yeast operator overlaps an upstream activation site. Cell 50(3):369-77
9)	Manney TR (1983) Expression of the BAR1 gene in Saccharomyces cerevisiae: induction by the alpha mating pheromone of an activity associated with a secreted protein. J Bacteriol 155(1):291-301
10)	Hagen DC, et al. (1991) Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol Cell Biol 11(6):2952-61
11)	MacKay VL, et al. (1988) The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin. Proc Natl Acad Sci U S A 85(1):55-9
12)	Egel-Mitani M, et al. (1990) A novel aspartyl protease allowing KEX2-independent MF alpha propheromone processing in yeast. Yeast 6(2):127-37