AVO1/YOL078W Summary 
| Standard Name | AVO1 |
|---|---|
| Systematic Name | YOL078W |
| Feature Type | ORF, Verified |
| Description | Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth (1, 2 and see Summary Paragraph) |
| Name Description | Adheres VOraciously (to TOR2) |
| Chromosomal Location | |
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| View Computational GO annotations for AVO1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Classical genetics | |
|---|---|
| null | |
| repressible | |
| Large-scale survey | |
| null | |
| overexpression | |
| reduction of function |
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| Resources |
| 47 total interaction(s) for 33 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Resources |
| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
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| Last Update | Coordinates: 2006-01-05 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000005438 |
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AVO1 encodes an essential protein that helps maintain the structural integrity of the TOR complex 2 (TORC2) (3). TORC2 is involved in regulating actin cytoskeleton polarization during cell cycle progression, cell wall integrity, and nutrient receptor endocytosis (4, 5). In addition to Avo1p, TORC2 contains Avo2p, Tsc11p, Lst8p, Bit61p, Slm1p, Slm2p, and Tor2p (1, 6). Experimental evidence suggests that TORC2 autophosphorylates sites on Avo1p but that this kinase function is not essential to TORC2 integrity (3).
Although the predicted molecular weight of Avo1p is 131 kDa, its apparent molecular weight is 165 kDa (1). Avo1p contains a Ras binding domain and its human homolog hSIN1 (mAvo1p) was originally isolated in a screen for human cDNAs that interfere with S. cerevisiae Ras signaling (7). Not only is Avo1p conserved from yeast to man, but TORC2 is also structurally conserved in higher eukaryotes and, like yeast TORC2, functions to regulate the actin cytoskeleton (1, 8).
Last updated: 2005-11-08 
| 1) | Loewith R, et al. (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10(3):457-68 |
| 2) | Wedaman KP, et al. (2003) Tor kinases are in distinct membrane-associated protein complexes in Saccharomyces cerevisiae. Mol Biol Cell 14(3):1204-20 |
| 3) | Wullschleger S, et al. (2005) Molecular organization of target of rapamycin complex 2. J Biol Chem 280(35):30697-704 |
| 4) | Schmidt A, et al. (1996) TOR2 is required for organization of the actin cytoskeleton in yeast. Proc Natl Acad Sci U S A 93(24):13780-5 |
| 5) | deHart AK, et al. (2003) Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell 14(11):4676-84 |
| 6) | Reinke A, et al. (2004) TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J Biol Chem 279(15):14752-62 |
| 7) | Colicelli J, et al. (1991) Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(7):2913-7 |
| 8) | Jacinto E, et al. (2004) Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol 6(11):1122-8 |
