ATP20/YPR020W Summary

Standard Name	ATP20 (see Nomenclature conflict Note)
Systematic Name	YPR020W
Feature Type	ORF, Verified
Description	Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae (1, 2, 3 and see Summary Paragraph)
Name Description	ATP synthase

Chromosomal Location	ChrXVI:599870 to 600217 \| ORF Map \| GBrowse

Gene Ontology Annotations All ATP20 GO evidence and references

	View Computational GO annotations for ATP20
Molecular Function
Manually curated	contributes_to proton-transporting ATPase activity, rotational mechanism (IDA) structural molecule activity (IMP, IPI)
Biological Process
Manually curated	ATP synthesis coupled proton transport (IDA, IMP, IPI, ISS) cristae formation (IMP) protein complex assembly (IMP) protein complex oligomerization (IMP)
Cellular Component
Manually curated	mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) (IMP, IPI, ISS)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All ATP20 Phenotype evidence and references

Classical genetics
null	mitochondrial morphology: abnormal respiratory growth: decreased rate respiratory growth: decreased
repressible	mitochondrial morphology: abnormal
Large-scale survey
null	competitive fitness: decreased haploinsufficient vacuolar morphology: abnormal viable
overexpression	filamentous growth: increased vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ATP20 Interaction evidence and references

	61 total interaction(s) for 45 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 3 Affinity Capture-Western: 3 Co-purification: 1 PCA: 2 Reconstituted Complex: 1 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 43 Positive Genetic: 5 Synthetic Lethality: 1 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ATP20 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:599870 to 600217 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..348	599870..600217	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000006224

NOMENCLATURE CONFLICT NOTE

Name	Relevance	Description
ATP19	Nomenclature conflict	Both ATP19/YOL077W-A, subunit k of mitochondrial ATP synthase, and ATP20/YPR020W, subunit g of mitochondrial ATP synthase, have been referred to as ATP19.

SUMMARY PARAGRAPH for ATP20

ATP20 encodes subunit g of mitochondrial ATP synthase (4). The ATP synthase complex utilizes proton motive force to generate ATP from ADP and P_i (5). The structure of this enzyme complex is highly conserved among diverse organisms and consists of two major components, soluble F₁ and membrane-bound F₀, each of which contains many subunits (6). Subunit g, like subunit e (Tim11p), is not essential for the basic function or assembly of the F₁/F₀ ATP synthase complex, but is required for complex dimerization and maximal enzyme stability. Deletion of ATP20 reduces the mitochondrial concentration of ATP synthase, but does not alter enzyme activity (1).

General ATP synthase structure and function are reviewed in references 5 and 7. For a review that is specific to yeast, see reference 6.

Last updated: 2001-01-17

References cited on this page View Complete Literature Guide for ATP20

1)	Arnold I, et al. (1998) Yeast mitochondrial F1F0-ATP synthase exists as a dimer: identification of three dimer-specific subunits. EMBO J 17(24):7170-8
2)	Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
3)	Davies KM, et al. (2012) Structure of the yeast F1Fo-ATP synthase dimer and its role in shaping the mitochondrial cristae. Proc Natl Acad Sci U S A 109(34):13602-7
4)	Boyle GM, et al. (1999) Identification of subunit g of yeast mitochondrial F1F0-ATP synthase, a protein required for maximal activity of cytochrome c oxidase. Eur J Biochem 262(2):315-23
5)	Boyer PD (1997) The ATP synthase--a splendid molecular machine. Annu Rev Biochem 66:717-49
6)	Devenish RJ, et al. (2000) Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex. Biochim Biophys Acta 1458(2-3):428-42
7)	Nakamoto RK, et al. (1999) Rotational coupling in the F0F1 ATP synthase. Annu Rev Biophys Biomol Struct 28:205-34