ATP20/YPR020W Summary Help

Standard Name ATP20
Systematic Name YPR020W
Feature Type ORF, Verified
Description Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae (1, 2, 3 and see Summary Paragraph)
Name Description ATP synthase
Chromosomal Location
ChrXVI:599870 to 600217 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ATP20 GO evidence and references
  View Computational GO annotations for ATP20
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Classical genetics
null
repressible
Large-scale survey
null
overexpression
Resources
62 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 3
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 43
  • Positive Genetic: 5
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 115
Molecular Weight (Da) 12,921
Isoelectric Point (pI) 10.66
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXVI:599870 to 600217 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..348 599870..600217 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006224
SUMMARY PARAGRAPH for ATP20

ATP20 encodes subunit g of mitochondrial ATP synthase (4). The ATP synthase complex utilizes proton motive force to generate ATP from ADP and Pi (5). The structure of this enzyme complex is highly conserved among diverse organisms and consists of two major components, soluble F1 and membrane-bound F0, each of which contains many subunits (6). Subunit g, like subunit e (Tim11p), is not essential for the basic function or assembly of the F1/F0 ATP synthase complex, but is required for complex dimerization and maximal enzyme stability. Deletion of ATP20 reduces the mitochondrial concentration of ATP synthase, but does not alter enzyme activity (1).

General ATP synthase structure and function are reviewed in references 5 and 7. For a review that is specific to yeast, see reference 6.

Last updated: 2001-01-17 Contact SGD

References cited on this page View Complete Literature Guide for ATP20
1) Arnold I, et al.  (1998) Yeast mitochondrial F1F0-ATP synthase exists as a dimer: identification of three dimer-specific subunits. EMBO J 17(24):7170-8
2) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
3) Davies KM, et al.  (2012) Structure of the yeast F1Fo-ATP synthase dimer and its role in shaping the mitochondrial cristae. Proc Natl Acad Sci U S A 109(34):13602-7
4) Boyle GM, et al.  (1999) Identification of subunit g of yeast mitochondrial F1F0-ATP synthase, a protein required for maximal activity of cytochrome c oxidase. Eur J Biochem 262(2):315-23
5) Boyer PD  (1997) The ATP synthase--a splendid molecular machine. Annu Rev Biochem 66:717-49
6) Devenish RJ, et al.  (2000) Insights into ATP synthase assembly and function through the molecular genetic manipulation of subunits of the yeast mitochondrial enzyme complex. Biochim Biophys Acta 1458(2-3):428-42
7) Nakamoto RK, et al.  (1999) Rotational coupling in the F0F1 ATP synthase. Annu Rev Biophys Biomol Struct 28():205-34