ASP3-3/YLR158C Summary Help

Standard Name ASP3-3
Systematic Name YLR158C
Alias ASP3
Feature Type ORF, Verified
Description Cell-wall L-asparaginase II involved in asparagine catabolism; expression induced during nitrogen starvation; ORF contains a short non-coding RNA that enhances expression of full-length gene; likely arose in via horizontal gene transfer from the wine yeast Wickerhamomyces anomalus or a close relative; reference strain S288C has four copies of ASP3; ASP3-3 has a paralog, ASP3-1, that arose from a segmental duplication (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description ASParaginase
Chromosomal Location
ChrXII:483637 to 482549 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ASP3-3 GO evidence and references
  View Computational GO annotations for ASP3-3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Large-scale survey
23 total interaction(s) for 22 unique genes/features.
Genetic Interactions
  • Negative Genetic: 21
  • Positive Genetic: 2

Expression Summary
Length (a.a.) 362
Molecular Weight (Da) 38,686
Isoelectric Point (pI) 4.53
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:483637 to 482549 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1089 483637..482549 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004148

ASP3 encodes a nitrogen catabolite-regulated cell wall L-asparaginase II (1). It is one of two asparaginases in yeast, the other being the constitutive intracellular asparaginase I Asp1p, with which Asp3p shares 46% identity (6). Asp3p catalyzes the conversion of L-asparagine to aspartate and ammonia. Asp3p is secreted in response to nitrogen starvation and appears to be regulated by Gln3p/Ure2p (7). ASP3 is located at the junction on chromosome XII with the rRNA genes and is repeated at least four times, to create ASP3-1, ASP3-2, ASP3-3, ASP3-4.

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for ASP3-3
1) Kim KW, et al.  (1988) Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene. J Biol Chem 263(24):11948-53
2) Oliveira EM, et al.  (2003) The role of the GATA factors Gln3p, Nil1p, Dal80p and the Ure2p on ASP3 regulation in Saccharomyces cerevisiae. Yeast 20(1):31-7
3) Katju V, et al.  (2009) Variation in gene duplicates with low synonymous divergence in Saccharomyces cerevisiae relative to Caenorhabditis elegans. Genome Biol 10(7):R75
4) Huang YC, et al.  (2010) Intragenic transcription of a noncoding RNA modulates expression of ASP3 in budding yeast. RNA 16(11):2085-93
5) League GP, et al.  (2012) The ASP3 locus in Saccharomyces cerevisiae originated by horizontal gene transfer from Wickerhamomyces. FEMS Yeast Res 12(7):859-63
6) Sinclair K, et al.  (1994) The ASP1 gene of Saccharomyces cerevisiae, encoding the intracellular isozyme of L-asparaginase. Gene 144(1):37-43
7) Bon EP, et al.  (1997) Asparaginase II of Saccharomyces cerevisiae. GLN3/URE2 regulation of a periplasmic enzyme. Appl Biochem Biotechnol 63-65():203-12