ASK1/YKL052C Summary Help

Standard Name ASK1 1
Systematic Name YKL052C
Feature Type ORF, Verified
Description Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; sumoylated in an Mms21p-dependent manner; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description Associated with Spindles and Kinetochores 1
Chromosomal Location
ChrXI:340546 to 339668 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ASK1 GO evidence and references
  View Computational GO annotations for ASK1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 10 genes
Classical genetics
Large-scale survey
reduction of function
91 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 22
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 13
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 18
  • PCA: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 5
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 6
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 292
Molecular Weight (Da) 32,071
Isoelectric Point (pI) 4.44
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXI:340546 to 339668 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 2008-06-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..879 340546..339668 2011-02-03 2008-06-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001535
References cited on this page View Complete Literature Guide for ASK1
1) Li Y, et al.  (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
2) Li Y and Elledge SJ  (2003) The DASH complex component Ask1 is a cell cycle-regulated Cdk substrate in Saccharomyces cerevisiae. Cell Cycle 2(2):143-8
3) Miranda JL, et al.  (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
4) Westermann S, et al.  (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
5) Westermann S, et al.  (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7) Yong-Gonzales V, et al.  (2012) The Smc5-Smc6 Complex Regulates Recombination at Centromeric Regions and Affects Kinetochore Protein Sumoylation during Normal Growth. PLoS One 7(12):e51540