ASF1/YJL115W Summary Help

Standard Name ASF1 1
Systematic Name YJL115W
Alias CIA1 2
Feature Type ORF, Verified
Description Nucleosome assembly factor; involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition; relocalizes to the cytosol in response to hypoxia (3, 4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description Anti-Silencing Function 9
Chromosomal Location
ChrX:196287 to 197126 | ORF Map | GBrowse
Gbrowse
Genetic position: -80.06 cM
Gene Ontology Annotations All ASF1 GO evidence and references
  View Computational GO annotations for ASF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Resources
Classical genetics
conditional
null
overexpression
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
815 total interaction(s) for 343 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 50
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 66
  • Biochemical Activity: 4
  • Co-crystal Structure: 3
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 5
  • Far Western: 1
  • PCA: 4
  • Protein-peptide: 1
  • Reconstituted Complex: 22
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 6
  • Dosage Lethality: 1
  • Dosage Rescue: 9
  • Negative Genetic: 245
  • Phenotypic Enhancement: 62
  • Phenotypic Suppression: 20
  • Positive Genetic: 33
  • Synthetic Growth Defect: 199
  • Synthetic Lethality: 67
  • Synthetic Rescue: 9

Resources
Expression Summary
histogram
Resources
Length (a.a.) 279
Molecular Weight (Da) 31,603
Isoelectric Point (pI) 3.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:196287 to 197126 | ORF Map | GBrowse
SGD ORF map
Genetic position: -80.06 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..840 196287..197126 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003651
SUMMARY PARAGRAPH for ASF1

Asf1p is involved in chromatin assembly throughout the cell cycle as well as disassembly of chromatin during transcription (10, 11, 12, 6). Specifically, ASF1 is required for chromatin assembly during DNA replication and DNA repair, establishment of silent chromatin, and reassembly of chromatin after transcription (3, 13, 14, 15, 12, 6). Asf1p stimulates nucleosome assembly by the chromatin assembly factor CAF-1 during DNA replication in vitro (15) and it interacts with Hir1p to promote heterochromatin formation (15, 16). Overexpression of Asf1p causes derepression of the silent mating type loci (9).

ASF1 has been shown to be involved in many aspects of chromatin metabolism during transcription. Interactions between Asf1p and a bromodomain protein, Bdf1p, that interacts with TFIID demonstrate a functional link between Asf1p and RNA polymerase II (17). ASF1 is required for chromatin reassembly after activator mediated transcription (6). In addition, Asf1p is involved in the eviction of histones during transcriptional activation (18, 6, 19). Cell lacking Asf1p display global changes in transcription similar to those seen in cells lacking Cac2p or histone H4 (20).

Chromatin assembly mediated by ASF1 may be subject to checkpoint regulation. Asf1p has been implicated as a target of the DNA damage checkpoint response by its interaction with Rad53p (14, 13). Additionally, asf1 mutants require functional replication checkpoint proteins for progression through S-phase (21).

Several copies of the MCB motif, a sequence motif commonly found upstream of genes transcribed in S-phase, are present upstream of ASF1 (9). ASF1 expression appears to be downregulated in conditions of oxygen deprivation (22)

Asf1p is highly conserved in flies and humans but the functions of S. cerevisiae Asf1p appear to be split between two human genes (3, 23, 24).

Last updated: 2006-10-05 Contact SGD

References cited on this page View Complete Literature Guide for ASF1
1) Sternglanz, R.  (1993) Personal Communication, Mortimer Map Edition 12
2) Yamaki M, et al.  (2001) Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1/CIA1. Genes Cells 6(12):1043-54
3) Tyler JK, et al.  (1999) The RCAF complex mediates chromatin assembly during DNA replication and repair. Nature 402(6761):555-60
4) Singer MS, et al.  (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
5) Osada S, et al.  (2001) The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. Genes Dev 15(23):3155-68
6) Schwabish MA and Struhl K  (2006) Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. Mol Cell 22(3):415-22
7) Nyswaner KM, et al.  (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214
8) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
9) Le S, et al.  (1997) Two new S-phase-specific genes from Saccharomyces cerevisiae. Yeast 13(11):1029-42
10) Robinson KM and Schultz MC  (2003) Replication-independent assembly of nucleosome arrays in a novel yeast chromatin reconstitution system involves antisilencing factor Asf1p and chromodomain protein Chd1p. Mol Cell Biol 23(22):7937-46
11) Green EM, et al.  (2005) Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol 15(22):2044-9
12) Adkins MW, et al.  (2004) Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes. Mol Cell 14(5):657-66
13) Emili A, et al.  (2001) Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1. Mol Cell 7(1):13-20
14) Hu F, et al.  (2001) Asf1 links Rad53 to control of chromatin assembly. Genes Dev 15(9):1061-6
15) Sharp JA, et al.  (2001) Yeast histone deposition protein Asf1p requires Hir proteins and PCNA for heterochromatic silencing. Curr Biol 11(7):463-73
16) Krawitz DC, et al.  (2002) Chromatin assembly factor I mutants defective for PCNA binding require Asf1/Hir proteins for silencing. Mol Cell Biol 22(2):614-25
17) Chimura T, et al.  (2002) Identification and characterization of CIA/ASF1 as an interactor of bromodomains associated with TFIID. Proc Natl Acad Sci U S A 99(14):9334-9
18) Adkins MW and Tyler JK  (2004) The histone chaperone Asf1p mediates global chromatin disassembly in vivo. J Biol Chem 279(50):52069-74
19) Korber P, et al.  (2006) The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters. J Biol Chem 281(9):5539-45
20) Zabaronick SR and Tyler JK  (2005) The histone chaperone anti-silencing function 1 is a global regulator of transcription independent of passage through S phase. Mol Cell Biol 25(2):652-60
21) Kats ES, et al.  (2006) Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants. Proc Natl Acad Sci U S A 103(10):3710-5
22) Lai LC, et al.  (2005) Dynamical remodeling of the transcriptome during short-term anaerobiosis in Saccharomyces cerevisiae: differential response and role of Msn2 and/or Msn4 and other factors in galactose and glucose media. Mol Cell Biol 25(10):4075-91
23) Munakata T, et al.  (2000) A human homologue of yeast anti-silencing factor has histone chaperone activity. Genes Cells 5(3):221-33
24) Tamburini BA, et al.  (2005) Functional conservation and specialization among eukaryotic anti-silencing function 1 histone chaperones. Eukaryot Cell 4(9):1583-90