ARG1/YOL058W Summary Help

Standard Name ARG1
Systematic Name YOL058W
Alias ARG10
Feature Type ORF, Verified
Description Arginosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate (1 and see Summary Paragraph)
Name Description ARGinine requiring
Chromosomal Location
ChrXV:219211 to 220473 | ORF Map | GBrowse
Genetic position: -56 cM
Gene Ontology Annotations All ARG1 GO evidence and references
  View Computational GO annotations for ARG1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 29 genes
Classical genetics
Large-scale survey
21 total interaction(s) for 18 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Co-purification: 1

Genetic Interactions
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 4

Expression Summary
Length (a.a.) 420
Molecular Weight (Da) 46,928
Isoelectric Point (pI) 5.25
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXV:219211 to 220473 | ORF Map | GBrowse
Genetic position: -56 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1263 219211..220473 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005419

ARG1 encodes argininosuccinate synthase (2), which catalyzes the eighth step in arginine biosynthesis (1). Arg1p also plays a catabolic role in the utilization of citrulline as a nitrogen source (3). Arg1p and other enzymes that catalyze steps in the part of the arginine biosynthetic pathway going from ornithine to arginine are cytosolic (1). Like other genes encoding arginine biosynthetic enzymes, ARG1 is transcriptionally repressed in the presence of arginine and is regulated by general amino acid control (2, 4). Arginine-responsive transcription factors, including Arg80p, Arg81p, Arg82p, and Mcm1p, have been identified (5, 6), as have their target upstream activating sequences in ARG1 (, 2, 7, 8, 4). Arg1p is similar to the E. coli argininosuccinate synthase (argG), and similar enzymes have been identifed in archaebacteria and mammals (9). Mutations in the human gene encoding argininosuccinate synthase cause citrullinemia(OMIM) (10, 11).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for ARG1
1) Jauniaux JC, et al.  (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
2) Crabeel M, et al.  (1988) Arginine repression of the Saccharomyces cerevisiae ARG1 gene. Comparison of the ARG1 and ARG3 control regions. Curr Genet 13(2):113-24
3) Hilger F, et al.  (1979) Yeast argininosuccinate synthetase. Purification; structural and kinetic properties. Eur J Biochem 94(1):153-63
4) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Messenguy F  (1987) Multiplicity of regulatory mechanisms controlling amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol Sci 4(5):150-3
6) Messenguy F and Dubois E  (1993) Genetic evidence for a role for MCM1 in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Cell Biol 13(4):2586-92
7) Crabeel M, et al.  (1990) Arginine-specific repression in Saccharomyces cerevisiae: kinetic data on ARG1 and ARG3 mRNA transcription and stability support a transcriptional control mechanism. Mol Cell Biol 10(3):1226-33
8) De Rijcke M, et al.  (1992) Characterization of the DNA target site for the yeast ARGR regulatory complex, a sequence able to mediate repression or induction by arginine. Mol Cell Biol 12(1):68-81
9) Van Vliet F, et al.  (1990) Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisiae: comparison with methanogenic archaebacteria and mammals. Gene 95(1):99-104
10) Kobayashi K, et al.  (1990) Heterogeneity of mutations in argininosuccinate synthetase causing human citrullinemia. J Biol Chem 265(19):11361-7
11) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10