APT2/YDR441C Summary

Standard Name	APT2 1
Systematic Name	YDR441C
Feature Type	ORF, Verified
Description	Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication (2, 3 and see Summary Paragraph)
Name Description	Adenine PhosphoribosylTransferase 1

Chromosomal Location	ChrIV:1345062 to 1344517 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All APT2 GO evidence and references

	View Computational GO annotations for APT2
Molecular Function
Manually curated	NOT adenine phosphoribosyltransferase activity (IDA) molecular_function unknown (ND)
Biological Process
Manually curated	biological_process unknown (ND)
Cellular Component
High-throughput	cytoplasm (IDA)

Pathways	salvage pathways of adenine, hypoxanthine, and their nucleosides salvage pathways of purines and their nucleosides superpathway of purine biosynthesis and salvage pathways

Mutant phenotypes All APT2 Phenotype evidence and references

Large-scale survey
null	competitive fitness: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All APT2 Interaction evidence and references

	26 total interaction(s) for 24 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Protein-RNA: 1 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 15 Synthetic Growth Defect: 2 Synthetic Lethality: 5 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All APT2 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrIV:1345062 to 1344517 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..546	1345062..1344517	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000002849

SUMMARY PARAGRAPH for APT2

APT2 is a gene with similarity to adenine phosphoribosyltransferase (APRT) (1). It is likely a pseudogene that arose via duplication of the APRT-encoding gene APT1 (). APT2 expressed in E. coli shows no APRT activity, and the gene is not expressed under normal physiological conditions in yeast (2).

Last updated: 1999-12-31

References cited on this page View Complete Literature Guide for APT2

1)	Yuryev A and Corden JL (1994) A Saccharomyces cerevisiae gene encoding a potential adenine phosphoribosyltransferase. Yeast 10(5):659-62
2)	Alfonzo JD, et al. (1999) APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae. J Bacteriol 181(1):347-52
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61