APM4/YOL062C Summary Help

Standard Name APM4
Systematic Name YOL062C
Alias AMP1
Feature Type ORF, Verified
Description Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals (1, 2, 3)
Name Description clathrin Adaptor Protein complex Medium chain
Chromosomal Location
ChrXV:211995 to 210520 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All APM4 GO evidence and references
  View Computational GO annotations for APM4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
35 total interaction(s) for 23 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 4
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 15
  • Positive Genetic: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 491
Molecular Weight (Da) 55,387
Isoelectric Point (pI) 8.36
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:211995 to 210520 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1476 211995..210520 2006-01-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005423
References cited on this page View Complete Literature Guide for APM4
1) Cowles CR, et al.  (1997) The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole. Cell 91(1):109-18
2) Yeung BG, et al.  (1999) Adaptor complex-independent clathrin function in yeast. Mol Biol Cell 10(11):3643-59
3) Chapa-Y-Lazo B, et al.  (2014) Yeast endocytic adaptor ap-2 binds the stress sensor mid2 and functions in polarized cell responses. Traffic 15(5):546-57