Standard Name	APL2 1
Systematic Name	YKL135C
Feature Type	ORF, Verified
Description	Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description	clathrin Adaptor Protein complex Large chain 1

Chromosomal Location	ChrXI:188640 to 186460 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for APL2
Molecular Function
Manually curated	clathrin binding (IPI, ISS)
Biological Process
Manually curated	Golgi to vacuole transport (IMP) vesicle-mediated transport (IPI, ISS)
Cellular Component
Manually curated	AP-1 adaptor complex (IPI, ISS)

Classical genetics
null	vacuolar morphology: abnormal vacuolar transport: abnormal
reduction of function	vacuolar transport: decreased
Large-scale survey
null	rubidium(1+) accumulation: decreased competitive fitness: decreased hyperosmotic stress resistance: decreased Fus-Mid-GFP distribution: abnormal resistance to arsenite(3-): decreased resistance to monensin: decreased resistance to sirolimus: decreased resistance to mefloquine: decreased resistance to glycolaldehyde: decreased resistance to hygromycin B: decreased resistance to wortmannin: normal toxin resistance: increased transposable element transposition: increased vacuolar morphology: abnormal viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	127 total interaction(s) for 73 unique genes/features.
Physical Interactions	Affinity Capture-MS: 40 Affinity Capture-RNA: 1 Affinity Capture-Western: 18 PCA: 3 Two-hybrid: 12
Genetic Interactions	Negative Genetic: 28 Phenotypic Enhancement: 1 Phenotypic Suppression: 3 Synthetic Growth Defect: 16 Synthetic Lethality: 2 Synthetic Rescue: 3
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXI:188640 to 186460 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2181 188640..186460 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000001618