APL2/YKL135C Summary

APL2 BASIC INFORMATION

Standard Name	APL2 1
Systematic Name	YKL135C
Feature Type	ORF, Verified
Description	Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting (1, 2, 3)
Name Description	clathrin Adaptor Protein complex Large chain 1

GO Annotations	All APL2 GO evidence and references
	View Computational GO annotations for APL2
Molecular Function
Manually curated	clathrin binding (IPI, ISS)
Biological Process
Manually curated	Golgi to vacuole transport (IMP) vesicle-mediated transport (IPI, ISS)
Cellular Component
Manually curated	AP-1 adaptor complex (IPI, ISS)

Mutant Phenotype	All APL2 Phenotype details and references
Classical genetics
null	vacuolar morphology: abnormal vacuolar transport: abnormal
reduction of function	vacuolar transport: decreased
Large-scale survey
null	competitive fitness: decreased hyperosmotic stress resistance: decreased resistance to rapamycin: decreased resistance to wortmannin: normal resistance to hygromycin B: decreased resistance to monensin: decreased toxin resistance: increased transposable element transposition: increased viable

Interactions	APL2 All interactions details and references
	79 total interaction(s) for 36 unique genes/features.
Physical Interactions	Affinity Capture-MS: 35 Affinity Capture-Western: 13 PCA: 3 Two-hybrid: 12
Genetic Interactions	Phenotypic Enhancement: 1 Phenotypic Suppression: 3 Synthetic Growth Defect: 10 Synthetic Lethality: 1 Synthetic Rescue: 1

Sequence Information

ChrXI:188645 to 186465 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-12-15 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2181	188645..186465	2005-12-15	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001618

APL2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for APL2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for APL2]

1)	Rad MR, et al. (1995) Saccharomyces cerevisiae Apl2p, a homologue of the mammalian clathrin AP beta subunit, plays a role in clathrin-dependent Golgi functions. J Cell Sci 108 ( Pt 4):1605-15
2)	Kirchhausen T (2000) Three ways to make a vesicle. Nat Rev Mol Cell Biol 1(3):187-98
3)	Yeung BG, et al. (1999) Adaptor complex-independent clathrin function in yeast. Mol Biol Cell 10(11):3643-59

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