APC5/YOR249C Summary Help

Standard Name APC5 1
Systematic Name YOR249C
Alias RMC1 2
Feature Type ORF, Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress (1, 3, 4, 5)
Name Description Anaphase Promoting Complex 1
Chromosomal Location
ChrXV:800731 to 798674 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All APC5 GO evidence and references
  View Computational GO annotations for APC5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
reduction of function
Large-scale survey
null
reduction of function
unspecified
Resources
236 total interaction(s) for 153 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 55
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 7
  • Biochemical Activity: 4
  • Co-crystal Structure: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Growth Defect: 5
  • Dosage Rescue: 11
  • Negative Genetic: 83
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 6
  • Positive Genetic: 14
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 8
  • Synthetic Rescue: 16

Resources
Expression Summary
histogram
Resources
Length (a.a.) 685
Molecular Weight (Da) 79,274
Isoelectric Point (pI) 4.94
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXV:800731 to 798674 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2058 800731..798674 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000005775
References cited on this page View Complete Literature Guide for APC5
1) Zachariae W, et al.  (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
2) Harkness TA, et al.  (2002) The ubiquitin-dependent targeting pathway in Saccharomyces cerevisiae plays a critical role in multiple chromatin assembly regulatory steps. Genetics 162(2):615-32
3) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
4) Buschhorn BA, et al.  (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13
5) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76