APC4/YDR118W Summary Help

Standard Name APC4 1
Systematic Name YDR118W
Feature Type ORF, Verified
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress (1, 2, 3, 4)
Name Description Anaphase Promoting Complex 1
Chromosomal Location
ChrIV:685882 to 687840 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All APC4 GO evidence and references
  View Computational GO annotations for APC4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
reduction of function
Resources
108 total interaction(s) for 29 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 69
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 20
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 11

Genetic Interactions
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 652
Molecular Weight (Da) 75,255
Isoelectric Point (pI) 5.64
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:685882 to 687840 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1959 685882..687840 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002525
References cited on this page View Complete Literature Guide for APC4
1) Zachariae W, et al.  (1998) Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science 279(5354):1216-9
2) Zachariae W and Nasmyth K  (1999) Whose end is destruction: cell division and the anaphase-promoting complex. Genes Dev 13(16):2039-58
3) Buschhorn BA, et al.  (2011) Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1. Nat Struct Mol Biol 18(1):6-13
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76