ALY2/YJL084C Summary Help

Standard Name ALY2 1, 2
Systematic Name YJL084C
Alias ART3 3
Feature Type ORF, Verified
Description Alpha arrestin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication (4, 5, 6, 7)
Name Description Arrestin-Like Yeast protein 1
Chromosomal Location
ChrX:278002 to 274862 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All ALY2 GO evidence and references
  View Computational GO annotations for ALY2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
83 total interaction(s) for 62 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 16
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Biochemical Activity: 23
  • Co-purification: 3
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 14
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 1,046
Molecular Weight (Da) 117,215
Isoelectric Point (pI) 6.9
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:278002 to 274862 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1997-07-27
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3141 278002..274862 2011-02-03 1997-07-27
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003620
References cited on this page View Complete Literature Guide for ALY2
1) O' Donnell A  (2006) personal communication
2) Gupta R, et al.  (2007) Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast. Mol Syst Biol 3:116
3) Lin CH, et al.  (2008) Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell 135(4):714-25
4) Shi XZ and Ao SZ  (2002) Analysis of phosphorylation of YJL084c, a yeast protein. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 34(4):433-8
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) O'Donnell AF, et al.  (2010) {alpha}-Arrestins Aly1 and Aly2 Regulate Intracellular Trafficking in Response to Nutrient Signaling. Mol Biol Cell 21(20):3552-66
7) Hatakeyama R, et al.  (2010) Endocytosis of the Aspartic Acid/Glutamic Acid Transporter Dip5 Is Triggered by Substrate-Dependent Recruitment of the Rsp5 Ubiquitin Ligase via the Arrestin-Like Protein Aly2. Mol Cell Biol 30(24):5598-607