ALG9/YNL219C Summary 
| Standard Name | ALG9 1 |
|---|---|
| Systematic Name | YNL219C |
| Feature Type | ORF, Verified |
| Description | Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation (1, 2, 3 and see Summary Paragraph) |
| Name Description | Asparagine-Linked Glycosylation 4 |
| Chromosomal Location | |
|---|---|
| Note: this feature is encoded on the Crick strand. | |
|
| View Computational GO annotations for ALG9 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
| Pathways |
|---|
| 287 total interaction(s) for 212 unique genes/features. | |
| Physical Interactions |
|
| Genetic Interactions |
|
| Resources |
|
|
| |
| Resources |
| Localization | |
|---|---|
| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
| Note: this feature is encoded on the Crick strand. | |||||||||||||
|
| |||||||||||||
| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
| ||||||||||||
| Retrieve sequences | |||||||||||||
| S288C only | |
|---|---|
| S288C vs. other species | |
| S288C vs. other strains |
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
|---|
| Primary SGDID | S000005163 |
|---|
During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 5, 6).
Alg9p, which is an alpha 1,2 mannosyltransferase, catalyzes two steps in lipid-linked oligosaccharide (LLO) assembly: the addition of the seventh and ninth mannose moieties to the growing oligosaccharide (1, 2) in the lumen of the endoplasmic reticulum. The sixth and eighth mannose moieties are added by Alg3p and Alg12p, respectively. Disruption of ALG9 causes accumulation of lipid-linked oligosaccharides with six mannose residues and hypoglycosylation of secreted proteins (1).
Human ALG9 (OMIM) cDNA complements the deletion of yeast ALG9 in a delta-alg9 wbp1-2 background (2) and has been found to be mutated in the
Last updated: 2005-07-12 
| 1) | Burda P, et al. (1996) Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALG9 gene encoding a putative mannosyl transferase. Proc Natl Acad Sci U S A 93(14):7160-5 |
| 2) | Frank CG, et al. (2004) Identification and functional analysis of a defect in the human ALG9 gene: definition of congenital disorder of glycosylation type IL. Am J Hum Genet 75(1):146-50 |
| 3) | Frank CG and Aebi M (2005) ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis. Glycobiology 15(11):1156-63 |
| 4) | Huffaker TC and Robbins PW (1982) Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J Biol Chem 257(6):3203-10 |
| 5) | Herscovics A and Orlean P (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50 |
| 6) | Burda P and Aebi M (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57 |
