ALG11/YNL048W Summary 
ALG11 BASIC INFORMATION
| Standard Name | ALG11 1 |
|---|---|
| Systematic Name | YNL048W |
| Feature Type | ORF, Verified |
| Description | Alpha-1,2-mannosyltransferase, catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER (1, 2 and see Summary Paragraph) 
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| Name Description | Asparagine-Linked Glycosylation 1 |
| GO Annotations | All ALG11 GO evidence and references |
|---|---|
| View Computational GO annotations for ALG11 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Pathways |
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| Mutant Phenotype | All ALG11 Phenotype details and references |
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| Classical genetics | |
| null |
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| Interactions | ALG11 All interactions details and references |
|---|---|
| 17 total interaction(s) for 16 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| External Links | All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000004993 |
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ALG11 RESOURCES
| SGD ORF map | GBrowse |
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for ALG11
SUMMARY PARAGRAPH for ALG11
During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 3, 4).
ALG11 encodes an alpha 1,2 mannosyltransferase that catalyzes two sequential steps in oligosaccharide-lipid intermediate assembly: the addition of the fourth and fifth mannose residues to growing lipid-linked oligosaccharides (LLO's) on the cytosolic side of the ER (2). Afterwards, Rft1p flips the LLO into the ER lumen, where it is extended further. Truncated alg11-1 LLO's with three or four mannose residues are flipped into the ER and partially extended, leading to the accumulation of LLO's with seven mannose residues. ALG11 is needed for normal growth at 25° C and is essential at 37° C (1). Alg11p and its closest homolog, Alg2p, each form complexes with Alg1p, but not with each other (5). ALG11 restores function to S. pombe mutants lacking the ALG11 homolog gmd3 (6).
Last updated: 2007-04-17
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ALG11]
| 1) | Cipollo JF, et al. (2001) The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum. J Biol Chem 276(24):21828-40 |
| 2) | O'Reilly MK, et al. (2006) In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis. Biochemistry 45(31):9593-603 |
| 3) | Herscovics A and Orlean P (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50 |
| 4) | Burda P and Aebi M (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57 |
| 5) | Gao XD, et al. (2004) Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum. Glycobiology 14(6):559-70 |
| 6) | Umeda K, et al. (2000) Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis. Yeast 16(14):1261-71 |
