ALG11/YNL048W Summary Help

Standard Name ALG11 1
Systematic Name YNL048W
Feature Type ORF, Verified
Description Alpha-1,2-mannosyltransferase; catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ER (1, 2 and see Summary Paragraph)
Name Description Asparagine-Linked Glycosylation 3
Chromosomal Location
ChrXIV:538173 to 539819 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ALG11 GO evidence and references
  View Computational GO annotations for ALG11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
repressible
Resources
21 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-Western: 1
  • PCA: 13

Genetic Interactions
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 548
Molecular Weight (Da) 63,143
Isoelectric Point (pI) 9.7
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIV:538173 to 539819 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1997-01-28
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1647 538173..539819 2011-02-03 1997-01-28
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004993
SUMMARY PARAGRAPH for ALG11

During N-linked glycosylation of proteins, oligosaccharide chains are assembled on the carrier molecule dolichyl pyrophosphate in the following order: 2 molecules of N-acetylglucosamine (GlcNAc), 9 molecules of mannose, and 3 molecules of glucose. These 14-residue oligosaccharide cores are then transferred to asparagine residues on nascent polypeptide chains in the endoplasmic reticulum (ER). As proteins progress through the Golgi apparatus, the oligosaccharide cores are modified by trimming and extension to generate a diverse array of glycosylated proteins (reviewed in 4, 5).

ALG11 encodes an alpha 1,2 mannosyltransferase that catalyzes two sequential steps in oligosaccharide-lipid intermediate assembly: the addition of the fourth and fifth mannose residues to growing lipid-linked oligosaccharides (LLO's) on the cytosolic side of the ER (2). Afterwards, Rft1p flips the LLO into the ER lumen, where it is extended further. Truncated alg11-1 LLO's with three or four mannose residues are flipped into the ER and partially extended, leading to the accumulation of LLO's with seven mannose residues. ALG11 is needed for normal growth at 25° C and is essential at 37° C (1). Alg11p and its closest homolog, Alg2p, each form complexes with Alg1p, but not with each other (6). ALG11 restores function to S. pombe mutants lacking the ALG11 homolog gmd3 (7).

Last updated: 2005-07-12 Contact SGD

References cited on this page View Complete Literature Guide for ALG11
1) Cipollo JF, et al.  (2001) The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum. J Biol Chem 276(24):21828-40
2) O'reilly MK, et al.  (2006) In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis. Biochemistry 45(31):9593-603
3) Huffaker TC and Robbins PW  (1982) Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. J Biol Chem 257(6):3203-10
4) Herscovics A and Orlean P  (1993) Glycoprotein biosynthesis in yeast. FASEB J 7(6):540-50
5) Burda P and Aebi M  (1999) The dolichol pathway of N-linked glycosylation. Biochim Biophys Acta 1426(2):239-57
6) Gao XD, et al.  (2004) Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum. Glycobiology 14(6):559-70
7) Umeda K, et al.  (2000) Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis. Yeast 16(14):1261-71