ALF1/YNL148C Summary Help

ALF1 BASIC INFORMATION

Standard Name ALF1
Systematic Name YNL148C
Feature Type ORF, Verified
Description Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance (1 and see Summary Paragraph)
Name Description ALpha-tubulin Foldin
GO Annotations All ALF1 GO evidence and references
    View Computational GO annotations for ALF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Mutant Phenotype All ALF1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions ALF1 All interactions details and references
21 total interaction(s) for 15 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 3
  • Two-hybrid: 3
Genetic Interactions
  • Dosage Lethality: 1
  • Phenotypic Enhancement: 5
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
Sequence Information
ChrXIV:350673 to 349909 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..765 350673..349909 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005092

ALF1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for ALF1

SUMMARY PARAGRAPH for ALF1

Microtubules are conserved cytoskeletal elements that form by the polymerization of alpha- and beta-tubulin heterodimers. The formation of polymerization-competent tubulin heterodimers requires that alpha-tubulin and beta-tubulin be properly folded. Specific cofactors are required for the folding of alpha- and beta-tubulin in vitro and homologs of these cofactors have been found in many organisms, including S. cerevisiae (reviewed in 2).

In S. cerevisiae, ALF1 is a non-essential gene that is homologous to mammalian cofactor B 3, 1. In vitro, cofactor B acts in the post-chaperonin folding of alpha-tubulin 3. Consistent with in vitro studies, Alf1p genetically acts upstream of Pac2p/cofactor E 3, 1. ALF1 genetically interacts with the other tubulin cofactors (CIN1/cofactor D, RBL2/cofactor A), and is essential in combination with specific alpha-tubulin mutants 3, 1. alf1 null mutants are super-sensitive to benomyl, a microtubule depolymerizing drug 1.

Alf1p interacts with alpha-tubulin in the yeast two-hybrid and immunoprecipitation assays 1. Alf1p and cofactor B both contain a single CLIP-170 domain, which is found in several microtubule-associated proteins and is required for the Alf1p-alpha-tubulin interaction 1. Alf1p binds to a face of alpha-tubulin distinct of that of beta-tubulin binding 1. Alf1p-GFP localizes to cytoplasmic microtubules, suggesting that Alf1p may play an additional role in microtubule maintenance 1.

Last updated: 2003-08-28

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ALF1]

1) Feierbach B, et al.  (1999) Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin. J Cell Biol 144(1):113-24
2) Lopez-Fanarraga M, et al.  (2001) Review: postchaperonin tubulin folding cofactors and their role in microtubule dynamics. J Struct Biol 135(2):219-29
3) Tian G, et al.  (1997) Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors. J Cell Biol 138(4):821-32