AIP1/YMR092C Summary Help

Standard Name AIP1 1
Systematic Name YMR092C
Feature Type ORF, Verified
Description Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress (2, 3, 4, 5)
Name Description Actin Interacting Protein 1
Chromosomal Location
ChrXIII:453479 to 451632 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All AIP1 GO evidence and references
  View Computational GO annotations for AIP1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
overexpression
repressible
Resources
62 total interaction(s) for 33 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Protein-peptide: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 25
  • Phenotypic Enhancement: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 615
Molecular Weight (Da) 67,325
Isoelectric Point (pI) 5.34
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXIII:453479 to 451632 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1848 453479..451632 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004698
References cited on this page View Complete Literature Guide for AIP1
1) Amberg DC, et al.  (1995) Defining protein interactions with yeast actin in vivo. Nat Struct Biol 2(1):28-35
2) Rodal AA, et al.  (1999) Aip1p interacts with cofilin to disassemble actin filaments. J Cell Biol 145(6):1251-64
3) Voegtli WC, et al.  (2003) The structure of Aip1p, a WD repeat protein that regulates Cofilin-mediated actin depolymerization. J Biol Chem 278(36):34373-9
4) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5) Michelot A, et al.  (2013) Actin filament elongation in Arp2/3-derived networks is controlled by three distinct mechanisms. Dev Cell 24(2):182-95