AGC1/YPR021C Summary

Standard Name	AGC1 1
Systematic Name	YPR021C
Feature Type	ORF, Verified
Description	Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis (1 and see Summary Paragraph)
Name Description	Aspartate-Glutamate Carrier 1

Chromosomal Location	ChrXVI:603357 to 600649 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All AGC1 GO evidence and references

	View Computational GO annotations for AGC1
Molecular Function
Manually curated	antiporter activity (IDA) L-aspartate transmembrane transporter activity (IDA) L-glutamate transmembrane transporter activity (IDA) uniporter activity (IDA)
Biological Process
Manually curated	aspartate transport (IDA) cellular nitrogen compound biosynthetic process (IMP) L-glutamate transport (IDA)
Cellular Component
Manually curated	mitochondrial inner membrane (ISS)
High-throughput	integral to membrane (ISM) mitochondrion (IDA)

Mutant phenotypes All AGC1 Phenotype evidence and references

Classical genetics
null	respiratory growth: absent respiratory growth: decreased rate respiratory growth: normal rate utilization of carbon source: absent viable
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to pentachlorophenol: decreased vacuolar morphology: abnormal
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All AGC1 Interaction evidence and references

	13 total interaction(s) for 11 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 PCA: 2
Genetic Interactions	Negative Genetic: 5 Synthetic Growth Defect: 4 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All AGC1 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXVI:603357 to 600649 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2709	603357..600649	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| TCDB \| UniProtKB

Primary SGDID	S000006225

SUMMARY PARAGRAPH for AGC1

AGC1 encodes a mitochondrial inner membrane aspartate/glutamate transporter that contributes to the biosynthesis of nitrogen compounds (1, 2). Agc1p is orthologous to the human proteins aralar (encoded by SLC25A12 (OMIM)) and citrin (encoded by SLC25A13 (OMIM)). Like the human orthologs, Agc1p can act as an aspartate-glutamate antiporter. In contrast to the human orthologs, Agc1p also functions as a uniporter for both glutamate and aspartate, it does not contain an N-terminal EF-hand Ca++-binding motif, and its transport activity is not Ca++ dependent (1).

In yeast, mutations in AGC1 result in decreased synthesis of valine, ornithine and citrulline, and the inability to grow on acetate or oleic acid. In humans, mutations in SLC25A13 are associated with type II citrullinemia (both adult-onset (OMIM) and neonatal-onset (OMIM1 and references therein).

Last updated: 2007-06-21

References cited on this page View Complete Literature Guide for AGC1

1)	Cavero S, et al. (2003) Identification and metabolic role of the mitochondrial aspartate-glutamate transporter in Saccharomyces cerevisiae. Mol Microbiol 50(4):1257-69
2)	Belenkiy R, et al. (2000) The yeast mitochondrial transport proteins: new sequences and consensus residues, lack of direct relation between consensus residues and transmembrane helices, expression patterns of the transport protein genes, and protein-protein interactions with other proteins. Biochim Biophys Acta 1467(1):207-18