AFT1/YGL071W Summary Help

Standard Name AFT1 1
Systematic Name YGL071W
Alias RCS1 2
Feature Type ORF, Verified
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress (3, 4, 5, 6, 7, 8)
Name Description Activator of Ferrous Transport 1
Chromosomal Location
ChrVII:372012 to 374084 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All AFT1 GO evidence and references
  View Computational GO annotations for AFT1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulatory Role
Binding motifs
    Regulatory modules predicted: stressResponse (529)
    Resources
    Mutant phenotypes All AFT1 Phenotype evidence and references
    Classical genetics
    activation
    null
    Large-scale survey
    null
    overexpression
    Resources
    interactions All AFT1 Interaction evidence and references
    290 total interaction(s) for 226 unique genes/features.
    Physical Interactions
    • Affinity Capture-MS: 3
    • Affinity Capture-RNA: 1
    • Affinity Capture-Western: 14
    • Protein-RNA: 1
    • Reconstituted Complex: 6
    • Two-hybrid: 17
    Genetic Interactions
    • Dosage Growth Defect: 31
    • Dosage Rescue: 2
    • Negative Genetic: 99
    • Phenotypic Enhancement: 6
    • Phenotypic Suppression: 3
    • Positive Genetic: 31
    • Synthetic Growth Defect: 59
    • Synthetic Lethality: 15
    • Synthetic Rescue: 2
    Resources
    Expression Summary
    histogram
    Resources
    Protein Information All AFT1 Protein evidence and references
    Localization
    Phosphorylation PhosphoGRID | PhosphoPep Database
    Structure
    Homologs
    sequence information
    ChrVII:372012 to 374084 | ORF Map | GBrowse
    SGD ORF map
    Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
    Subfeature details
    Relative
    Coordinates    		 Chromosomal
    Coordinates    		 Most Recent Updates
    Coordinates Sequence
    CDS 1..2073 372012..374084 2011-02-03 1996-07-31
    Retrieve sequences
    Analyze Sequence
    S288C only
    S288C vs. other species
    S288C vs. other strains
    Resources
    External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
    Primary SGDIDS000003039
    References cited on this page View Complete Literature Guide for AFT1
    1) Yamaguchi-Iwai Y, et al.  (1995) AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae. EMBO J 14(6):1231-9
    2) Gil R, et al.  (1991) RCS1, a gene involved in controlling cell size in Saccharomyces cerevisiae. Yeast 7(1):1-14
    3) Yamaguchi-Iwai Y, et al.  (1996) Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. EMBO J 15(13):3377-84
    4) Shakoury-Elizeh M, et al.  (2004) Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell 15(3):1233-43
    5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
    6) Pujol-Carrion N, et al.  (2006) Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae. J Cell Sci 119(Pt 21):4554-64
    7) Li H, et al.  (2011) Histidine 103 in Fra2 Is an Iron-Sulfur Cluster Ligand in the [2Fe-2S] Fra2-Grx3 Complex and Is Required for in Vivo Iron Signaling in Yeast. J Biol Chem 286(1):867-76
    8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
    9) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66