AFT1/YGL071W Summary Help

Standard Name AFT1 1
Systematic Name YGL071W
Alias RCS1 2
Feature Type ORF, Verified
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress (3, 4, 5, 6, 7, 8, 9)
Name Description Activator of Ferrous Transport 1
Chromosomal Location
ChrVII:372012 to 374084 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All AFT1 GO evidence and references
  View Computational GO annotations for AFT1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 132 genes
Regulators 4 genes
Resources
Classical genetics
activation
null
overexpression
Large-scale survey
null
overexpression
Resources
295 total interaction(s) for 229 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 14
  • Protein-RNA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Growth Defect: 31
  • Dosage Rescue: 2
  • Negative Genetic: 99
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 31
  • Synthetic Growth Defect: 60
  • Synthetic Lethality: 15
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 690
Molecular Weight (Da) 77,682
Isoelectric Point (pI) 6.88
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:372012 to 374084 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2073 372012..374084 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003039
References cited on this page View Complete Literature Guide for AFT1
1) Yamaguchi-Iwai Y, et al.  (1995) AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae. EMBO J 14(6):1231-9
2) Gil R, et al.  (1991) RCS1, a gene involved in controlling cell size in Saccharomyces cerevisiae. Yeast 7(1):1-14
3) Yamaguchi-Iwai Y, et al.  (1996) Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. EMBO J 15(13):3377-84
4) Shakoury-Elizeh M, et al.  (2004) Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell 15(3):1233-43
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Pujol-Carrion N, et al.  (2006) Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae. J Cell Sci 119(Pt 21):4554-64
7) Li H, et al.  (2011) Histidine 103 in Fra2 Is an Iron-Sulfur Cluster Ligand in the [2Fe-2S] Fra2-Grx3 Complex and Is Required for in Vivo Iron Signaling in Yeast. J Biol Chem 286(1):867-76
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Ueta R, et al.  (2012) Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Mol Cell Biol 32(24):4998-5008
10) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66