AFT1/YGL071W Summary Help

Standard Name AFT1 1
Systematic Name YGL071W
Alias RCS1 2
Feature Type ORF, Verified
Description Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress (3, 4, 5, 6, 7, 8, 9)
Name Description Activator of Ferrous Transport 1
Chromosomal Location
ChrVII:372012 to 374084 | ORF Map | GBrowse
Gene Ontology Annotations All AFT1 GO evidence and references
  View Computational GO annotations for AFT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 132 genes
Regulators 4 genes
Classical genetics
Large-scale survey
295 total interaction(s) for 229 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 14
  • Protein-RNA: 1
  • Reconstituted Complex: 6
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Growth Defect: 31
  • Dosage Rescue: 2
  • Negative Genetic: 99
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 3
  • Positive Genetic: 31
  • Synthetic Growth Defect: 60
  • Synthetic Lethality: 15
  • Synthetic Rescue: 4

Expression Summary
Length (a.a.) 690
Molecular Weight (Da) 77,682
Isoelectric Point (pI) 6.88
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:372012 to 374084 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2073 372012..374084 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003039
References cited on this page View Complete Literature Guide for AFT1
1) Yamaguchi-Iwai Y, et al.  (1995) AFT1: a mediator of iron regulated transcriptional control in Saccharomyces cerevisiae. EMBO J 14(6):1231-9
2) Gil R, et al.  (1991) RCS1, a gene involved in controlling cell size in Saccharomyces cerevisiae. Yeast 7(1):1-14
3) Yamaguchi-Iwai Y, et al.  (1996) Iron-regulated DNA binding by the AFT1 protein controls the iron regulon in yeast. EMBO J 15(13):3377-84
4) Shakoury-Elizeh M, et al.  (2004) Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell 15(3):1233-43
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Pujol-Carrion N, et al.  (2006) Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae. J Cell Sci 119(Pt 21):4554-64
7) Li H, et al.  (2011) Histidine 103 in Fra2 Is an Iron-Sulfur Cluster Ligand in the [2Fe-2S] Fra2-Grx3 Complex and Is Required for in Vivo Iron Signaling in Yeast. J Biol Chem 286(1):867-76
8) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
9) Ueta R, et al.  (2012) Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Mol Cell Biol 32(24):4998-5008
10) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66