AFR1/YDR085C Summary Help

Standard Name AFR1 1
Systematic Name YDR085C
Feature Type ORF, Verified
Description Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6)
Name Description Alpha-Factor Receptor regulator 7
Chromosomal Location
ChrIV:616150 to 614288 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 47.97 cM
Gene Ontology Annotations All AFR1 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 11 genes
Resources
Classical genetics
null
reduction of function
Large-scale survey
null
overexpression
Resources
25 total interaction(s) for 19 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1
  • FRET: 2
  • Protein-RNA: 1
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 1
  • Negative Genetic: 2
  • Positive Genetic: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 620
Molecular Weight (Da) 71,363
Isoelectric Point (pI) 9.23
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:616150 to 614288 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 47.97 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1863 616150..614288 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002492
References cited on this page View Complete Literature Guide for AFR1
1) Konopka JB  (1993) AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation. Mol Cell Biol 13(11):6876-88
2) DeMattei CR, et al.  (2000) Point mutations identify a conserved region of the saccharomyces cerevisiae AFR1 gene that is essential for both the pheromone signaling and morphogenesis functions. Genetics 155(1):43-55
3) Giot L and Konopka JB  (1997) Functional analysis of the interaction between Afr1p and the Cdc12p septin, two proteins involved in pheromone-induced morphogenesis. Mol Biol Cell 8(6):987-98
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Changwei Z, et al.  (2007) Afr1p has a role in regulating the localization of Mpk1p at the shmoo tip in Saccharomyces cerevisiae. FEBS Lett 581(14):2670-4
6) Bharucha JP, et al.  (2008) Saccharomyces cerevisiae Afr1 protein is a protein phosphatase 1/Glc7-targeting subunit that regulates the septin cytoskeleton during mating. Eukaryot Cell 7(8):1246-55
7) Davis C, et al.  (1998) Afr1p regulates the Saccharomyces cerevisiae alpha-factor receptor by a mechanism that is distinct from receptor phosphorylation and endocytosis. Genetics 148(2):625-35