Standard Name	AFI1 1
Systematic Name	YOR129C
Feature Type	ORF, Verified
Description	Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p (1, 2)
Name Description	ArF3-Interacting protein 1

Chromosomal Location	ChrXV:569558 to 566877 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for AFI1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	actin cortical patch localization (IGI, IMP) asymmetric protein localization (IMP) cellular bud site selection (IMP)
Cellular Component
Manually curated	cellular bud neck (IDA) nucleus (IGI, IMP) plasma membrane (IDA, IMP)
High-throughput	cytoplasm (IDA)

Classical genetics
null	actin cytoskeleton morphology: abnormal axial budding pattern: decreased Arf3p-GFP distribution: decreased viable
Large-scale survey
null	anaerobic growth: decreased competitive fitness: decreased vacuolar morphology: abnormal viable
overexpression	resistance to sirolimus: increased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	12 total interaction(s) for 7 unique genes/features.
Physical Interactions	Affinity Capture-Western: 1 Co-localization: 1 Protein-RNA: 1 Reconstituted Complex: 1 Two-hybrid: 4
Genetic Interactions	Negative Genetic: 1 Synthetic Growth Defect: 1 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DRYGIN | GeneMANIA | InterologFinder | YeastRC Two-Hybrid

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXV:569558 to 566877 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2682 569558..566877 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000005655