AFI1/YOR129C Summary

AFI1 BASIC INFORMATION

Standard Name	AFI1 1
Systematic Name	YOR129C
Feature Type	ORF, Verified
Description	Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p (1, 2)
Name Description	ArF3-Interacting protein 1

GO Annotations	All AFI1 GO evidence and references
	View Computational GO annotations for AFI1
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	actin cortical patch localization (IGI, IMP) asymmetric protein localization (IMP) cellular bud site selection (IMP)
Cellular Component
Manually curated	cellular bud neck (IDA) nucleus (IGI, IMP) plasma membrane (IDA, IMP)
High-throughput	cytoplasm (IDA)

Mutant Phenotype	All AFI1 Phenotype details and references
Classical genetics
null	actin cytoskeleton morphology: abnormal axial budding pattern: decreased Arf3p-GFP distribution: decreased viable
Large-scale survey
null	viable
overexpression	resistance to rapamycin: increased

Interactions	AFI1 All interactions details and references
	9 total interaction(s) for 4 unique genes/features.
Physical Interactions	Affinity Capture-Western: 1 Co-localization: 1 Protein-RNA: 1 Reconstituted Complex: 1 Two-hybrid: 4
Genetic Interactions	Phenotypic Enhancement: 1

Sequence Information

ChrXV:569559 to 566878 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2006-01-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2682	569559..566878	2006-01-05	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000005655

AFI1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for AFI1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for AFI1]

1)	Tsai PC, et al. (2008) Afi1p Functions as an Arf3p Polarization-specific Docking Factor for Development of Polarity. J Biol Chem 283(24):16915-27
2)	Wysocka M, et al. (2003) YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae. Acta Biochim Pol 50(3):883-90

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