AFG3/YER017C Summary

AFG3/YER017C Summary

Standard Name	AFG3 1
Systematic Name	YER017C
Alias	YTA10 2
Feature Type	ORF, Verified
Description	Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging (3, 4, 5)
Name Description	ATPase Family Gene 1

Chromosomal Location	ChrV:191788 to 189503 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All AFG3 GO evidence and references

	View Computational GO annotations for AFG3
Molecular Function
Manually curated	ATPase activity (IDA, IMP, ISS) metallopeptidase activity (IMP) protein binding (IPI)
Biological Process
Manually curated	cytoplasmic translation (IMP) protein complex assembly (IMP) protein import into mitochondrial intermembrane space (TAS) proteolysis (IMP) replicative cell aging (IMP) signal peptide processing (IMP)
Cellular Component
Manually curated	m-AAA complex (IDA) mitochondrial inner boundary membrane (IDA) mitochondrial inner membrane (IDA)
High-throughput	mitochondrion (IDA)

Mutant phenotypes All AFG3 Phenotype evidence and references

Classical genetics
null	phosphatidylethanolamine accumulation: decreased cardiolipin accumulation: decreased chronological lifespan: decreased heat sensitivity: decreased oxidative stress resistance: increased Ccp1-GFP accumulation: decreased Gcn4p-GFP accumulation: increased MrpL32 accumulation: decreased replicative lifespan: increased resistance to methyl methanesulfonate: decreased resistance to tunicamycin: increased
reduction of function	respiratory growth: absent
Large-scale survey
null	cell cycle progression in G1 phase: delayed proton accumulation: decreased ornithine accumulation: decreased glycogen accumulation: decreased competitive fitness: decreased dessication resistance: decreased haploinsufficient heat sensitivity: increased mitochondrial morphology: abnormal replicative lifespan: increased resistance to 2,4-dichlorophenol: decreased resistance to hygromycin B: decreased resistance to trichothecene: increased resistance to edelfosine: decreased resistance to CTBT: decreased resistance to doxorubicin: decreased resistance to amiodarone: decreased resistance to spermine: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: increased toxin resistance: increased vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All AFG3 Interaction evidence and references

	108 total interaction(s) for 76 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 1 Affinity Capture-Western: 14 Co-crystal Structure: 1 Co-fractionation: 1 Co-purification: 1 Two-hybrid: 1
Genetic Interactions	Dosage Rescue: 3 Negative Genetic: 46 Phenotypic Enhancement: 5 Phenotypic Suppression: 3 Positive Genetic: 12 Synthetic Growth Defect: 8 Synthetic Lethality: 2 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All AFG3 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrV:191788 to 189503 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2286	191788..189503	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000819

References cited on this page View Complete Literature Guide for AFG3

1)	Guelin E, et al. (1994) Sequence of the AFG3 gene encoding a new member of the FtsH/Yme1/Tma subfamily of the AAA-protein family. Yeast 10(10):1389-94
2)	Schnall R, et al. (1994) Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex. Yeast 10(9):1141-55
3)	Arlt H, et al. (1996) The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria. Cell 85(6):875-85
4)	Arlt H, et al. (1998) The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease. EMBO J 17(16):4837-47
5)	Delaney JR, et al. (2013) Stress profiling of longevity mutants identifies Afg3 as a mitochondrial determinant of cytoplasmic mRNA translation and aging. Aging Cell 12(1):156-66