Standard Name	AFG2
Systematic Name	YLR397C
Alias	DRG1
Feature Type	ORF, Verified
Description	ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs (1, 2, 3, 4)
Name Description	ATPase Family Gene 1

Chromosomal Location	ChrXII:914892 to 912550 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for AFG2
Molecular Function
Manually curated	ATPase activity (IDA)
Biological Process
Manually curated	response to drug (IMP) ribosomal large subunit biogenesis (IMP)
Cellular Component
Manually curated	intracellular (IDA) preribosome, large subunit precursor (IDA, IMP)

Classical genetics
conditional	heat sensitivity: decreased Sqt1p accumulation: decreased Bud20p-GFP distribution: abnormal
null	inviable
reduction of function	resistance to diazaborine: increased
repressible	nuclear export: decreased 60S ribosomal subunit accumulation: decreased
Large-scale survey
null	inviable
reduction of function	competitive fitness: increased
Resources	PROPHECY | PhenoM | SCMD | ScreenTroll | Yeast Fitness Database

	31 total interaction(s) for 24 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-Western: 5 Biochemical Activity: 2 PCA: 2 Two-hybrid: 6
Genetic Interactions	Negative Genetic: 7 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:914892 to 912550 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2343 914892..912550 2004-02-05 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004389