AFG2/YLR397C Summary Help

Standard Name AFG2 1
Systematic Name YLR397C
Alias DRG1
Feature Type ORF, Verified
Description ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs (1, 2, 3, 4, 5)
Name Description ATPase Family Gene 1
Chromosomal Location
ChrXII:914892 to 912550 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All AFG2 GO evidence and references
  View Computational GO annotations for AFG2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
null
reduction of function
repressible
Large-scale survey
null
reduction of function
repressible
unspecified
Resources
35 total interaction(s) for 28 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 11
  • Affinity Capture-Western: 6
  • Biochemical Activity: 2
  • PCA: 2
  • Two-hybrid: 6

Genetic Interactions
  • Negative Genetic: 7
  • Synthetic Lethality: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 780
Molecular Weight (Da) 84,747
Isoelectric Point (pI) 5.5
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:914892 to 912550 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2004-02-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2343 914892..912550 2004-02-05 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004389
References cited on this page View Complete Literature Guide for AFG2
1) Thorsness PE, et al.  (1993) AFG2, an essential gene in yeast, encodes a new member of the Sec18p, Pas1p, Cdc48p, TBP-1 family of putative ATPases. Yeast 9(11):1267-71
2) Zakalskiy A, et al.  (2002) Structural and enzymatic properties of the AAA protein Drg1p from Saccharomyces cerevisiae. Decoupling of intracellular function from ATPase activity and hexamerization. J Biol Chem 277(30):26788-95
3) Pertschy B, et al.  (2007) Cytoplasmic Recycling of 60S Preribosomal Factors Depends on the AAA Protein Drg1. Mol Cell Biol 27(19):6581-92
4) Kappel L, et al.  (2012) Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation. J Cell Biol 199(5):771-82
5) Loibl M, et al.  (2014) The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1. J Biol Chem 289(7):3913-22