ADR1/YDR216W Summary Help

Standard Name ADR1
Systematic Name YDR216W
Feature Type ORF, Verified
Description Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization (1, 2 and see Summary Paragraph)
Name Description Alcohol Dehydrogenase Regulator
Chromosomal Location
ChrIV:895035 to 899006 | ORF Map | GBrowse
Gbrowse
Genetic position: 125 cM
Gene Ontology Annotations All ADR1 GO evidence and references
  View Computational GO annotations for ADR1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Targets 19 genes
Regulators 2 genes
Resources
Classical genetics
overexpression
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
117 total interaction(s) for 99 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Co-localization: 2
  • Two-hybrid: 7

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 3
  • Negative Genetic: 31
  • Phenotypic Enhancement: 8
  • Phenotypic Suppression: 12
  • Positive Genetic: 8
  • Synthetic Growth Defect: 5
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,323
Molecular Weight (Da) 150,939
Isoelectric Point (pI) 6.71
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrIV:895035 to 899006 | ORF Map | GBrowse
SGD ORF map
Genetic position: 125 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3972 895035..899006 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000002624
SUMMARY PARAGRAPH for ADR1

ADR1 encodes a transcriptional activator involved in the expression of genes that are regulated by glucose repression (2). Adr1p was first identified as a transcription factor mediating expression of the alcohol dehydrogenase gene ADH2 (3), but it is now known to also activate genes involved glucose fermentation (e.g., ALD4 and ALD6), glycerol metabolism (e.g., GUT1 and GUT2), fatty acid utilization (e.g., POX1, FOX2), and peroxisome biogenesis (e.g., PEX1) (4, 5). Adr1p binds as a monomer to two half-sites (consensus sequence 5-TTGGRG-3) of the palindromic UAS1 element in the promoters of these genes (6, 7). Overexpression or deletion of ADR1 strongly deregulates the glucose response of Adr1p target genes (2, 8).

The Adr1p DNA binding domain (amino acids 84-160) consists of two Cys2His2 zinc fingers and an N-terminal proximal accessory region, which provides additional DNA contacts required for high-affinity binding (9 and references contained therein). Adr1p also contains four transcriptional activation domains (amino acids 76-172, 263-359, 420-462, 642-704, respectively) that are functionally redundant and interact with transcription factors such as Sua7p, TFIID, Ada2p, and Gcn5p (10, 11, 12).

Chromatin modification is required for and affected by Adr1p binding; loss of histone deacetylase activity allows Adr1p to bind promoters under repressing conditions, and Adr1p has been shown to mediate nucleosome repositioning in the ADH2 promoter (13, 14). The protein kinase Snf1p positively regulates Adr1p binding in the absence of glucose while the type 1 protein phosphatase (PP1) complex Glc7p/Reg1p inhibits the ability of Adr1p to bind DNA in the presence of glucose (15). The PP1 complex also affects ADR1 transcription levels and Adr1p activity through post-translational modification (16). Direct phosphorylation by protein kinases, such as cAMP-dependent protein kinase, influences Adr1p expression and function as well (17, 18).

Last updated: 2005-12-12 Contact SGD

References cited on this page View Complete Literature Guide for ADR1
1) Denis CL and Young ET  (1983) Isolation and characterization of the positive regulatory gene ADR1 from Saccharomyces cerevisiae. Mol Cell Biol 3(3):360-70
2) Young ET, et al.  (2003) Multiple pathways are co-regulated by the protein kinase Snf1 and the transcription factors Adr1 and Cat8. J Biol Chem 278(28):26146-58
3) Ciriacy M  (1975) Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. II. Two loci controlling synthesis of the glucose-repressible ADH II. Mol Gen Genet 138(2):157-64
4) Tachibana C, et al.  (2005) Combined global localization analysis and transcriptome data identify genes that are directly coregulated by Adr1 and Cat8. Mol Cell Biol 25(6):2138-46
5) Simon M, et al.  (1991) The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins. Mol Cell Biol 11(2):699-704
6) Thukral SK, et al.  (1991) Two monomers of yeast transcription factor ADR1 bind a palindromic sequence symmetrically to activate ADH2 expression. Mol Cell Biol 11(3):1566-77
7) Cheng C, et al.  (1994) Identification of potential target genes for Adr1p through characterization of essential nucleotides in UAS1. Mol Cell Biol 14(6):3842-52
8) Denis CL  (1987) The effects of ADR1 and CCR1 gene dosage on the regulation of the glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae. Mol Gen Genet 208(1-2):101-6
9) Schaufler LE and Klevit RE  (2003) Mechanism of DNA binding by the ADR1 zinc finger transcription factor as determined by SPR. J Mol Biol 329(5):931-9
10) Cook WJ, et al.  (1994) Dissection of the ADR1 protein reveals multiple, functionally redundant activation domains interspersed with inhibitory regions: evidence for a repressor binding to the ADR1c region. Mol Cell Biol 14(1):629-40
11) Chiang YC, et al.  (1996) ADR1 activation domains contact the histone acetyltransferase GCN5 and the core transcriptional factor TFIIB. J Biol Chem 271(50):32359-65
12) Komarnitsky PB, et al.  (1998) ADR1-mediated transcriptional activation requires the presence of an intact TFIID complex. Mol Cell Biol 18(10):5861-7
13) Verdone L, et al.  (2002) Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions. EMBO J 21(5):1101-11
14) Di Mauro E, et al.  (2002) In vivo changes of nucleosome positioning in the pretranscription state. J Biol Chem 277(9):7002-9
15) Young ET, et al.  (2002) Snf1 protein kinase regulates Adr1 binding to chromatin but not transcription activation. J Biol Chem 277(41):38095-103
16) Dombek KM, et al.  (1993) ADH2 expression is repressed by REG1 independently of mutations that alter the phosphorylation of the yeast transcription factor ADR1. Mol Cell Biol 13(7):4391-9
17) Dombek KM and Young ET  (1997) Cyclic AMP-dependent protein kinase inhibits ADH2 expression in part by decreasing expression of the transcription factor gene ADR1. Mol Cell Biol 17(3):1450-8
18) Cherry JR, et al.  (1989) Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1. Cell 56(3):409-19
19) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87