ADD66/YKL206C Summary

ADD66 BASIC INFORMATION

Standard Name	ADD66 1
Systematic Name	YKL206C
Alias	PBA2 2 , POC2 3
Feature Type	ORF, Verified
Description	Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly (2, 4)
Name Description	Alpha1-proteinase inhibitor-Degradation Deficient 1

GO Annotations	All ADD66 GO evidence and references
	View Computational GO annotations for ADD66
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	ER-associated protein catabolic process (IMP) proteasome assembly (IGI, IMP, ISS)
Cellular Component
Manually curated	cytosol (IDA)
High-throughput	cytoplasm (IDA)

Mutant Phenotype	All ADD66 Phenotype details and references
Classical genetics
null	metal resistance: decreased resistance to 4-nitroquinoline N-oxide: increased resistance to canavanine: normal resistance to methyl methanesulfonate: increased resistance to camptothecin: increased
Large-scale survey
null	resistance to ethanol: decreased viable

Interactions	ADD66 All interactions details and references
	21 total interaction(s) for 16 unique genes/features.
Physical Interactions	Affinity Capture-MS: 8 Affinity Capture-RNA: 1 Affinity Capture-Western: 3 Biochemical Activity: 3 Co-fractionation: 2
Genetic Interactions	Synthetic Growth Defect: 3 Synthetic Rescue: 1

Sequence Information

ChrXI:49810 to 49007 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-02-13 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..804	49810..49007	2004-02-13	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000001689

ADD66 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for ADD66

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ADD66]

1)	Palmer EA, et al. (2003) Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation. J Cell Sci 116(Pt 11):2361-73
2)	Li X, et al. (2007) beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint. EMBO J 26(9):2339-49
3)	Le Tallec B, et al. (2007) 20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals. Mol Cell 27(4):660-74
4)	Scott CM, et al. (2007) ADD66, a Gene Involved in the Endoplasmic Reticulum-associated Degradation of {alpha}-1-Antitrypsin-Z in Yeast, Facilitates Proteasome Activity and Assembly. Mol Biol Cell 18(10):3776-87

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