ADD66/YKL206C Summary Help

Standard Name ADD66 1
Systematic Name YKL206C
Alias PBA2 2 , POC2 3
Feature Type ORF, Verified
Description Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly (2, 4, 5)
Name Description Alpha1-proteinase inhibitor-Degradation Deficient 1
Chromosomal Location
ChrXI:49809 to 49006 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ADD66 GO evidence and references
  View Computational GO annotations for ADD66
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
46 total interaction(s) for 36 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 3
  • Biochemical Activity: 3
  • Co-crystal Structure: 1
  • Co-fractionation: 2
  • Reconstituted Complex: 1

Genetic Interactions
  • Negative Genetic: 6
  • Positive Genetic: 9
  • Synthetic Growth Defect: 7
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 267
Molecular Weight (Da) 30,739
Isoelectric Point (pI) 4.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXI:49809 to 49006 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..804 49809..49006 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000001689
References cited on this page View Complete Literature Guide for ADD66
1) Palmer EA, et al.  (2003) Differential requirements of novel A1PiZ degradation deficient (ADD) genes in ER-associated protein degradation. J Cell Sci 116(Pt 11):2361-73
2) Li X, et al.  (2007) beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint. EMBO J 26(9):2339-49
3) Le Tallec B, et al.  (2007) 20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals. Mol Cell 27(4):660-74
4) Scott CM, et al.  (2007) ADD66, a Gene Involved in the Endoplasmic Reticulum-associated Degradation of {alpha}-1-Antitrypsin-Z in Yeast, Facilitates Proteasome Activity and Assembly. Mol Biol Cell 18(10):3776-87
5) Stadtmueller BM, et al.  (2012) Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. J Biol Chem 287(44):37371-82