HFI1/YPL254W Summary Help

Standard Name HFI1
Systematic Name YPL254W
Alias ADA1 1 , SUP110 , SRM12 2 , GAN1 3
Feature Type ORF, Verified
Description Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions (4, 5, 6, 7, 8)
Name Description Histone H2A Functional Interactor
Chromosomal Location
ChrXVI:69485 to 70951 | ORF Map | GBrowse
Gene Ontology Annotations All HFI1 GO evidence and references
  View Computational GO annotations for HFI1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 406 genes
Regulators 4 genes
Classical genetics
Large-scale survey
312 total interaction(s) for 158 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 144
  • Affinity Capture-Western: 26
  • Biochemical Activity: 4
  • Co-purification: 5
  • Reconstituted Complex: 2
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Negative Genetic: 71
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 16
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 28
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 488
Molecular Weight (Da) 54,466
Isoelectric Point (pI) 5.47
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:69485 to 70951 | ORF Map | GBrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1467 69485..70951 1996-07-31 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006175
References cited on this page View Complete Literature Guide for HFI1
1) Berger SL, et al.  (1992) Genetic isolation of ADA2: a potential transcriptional adaptor required for function of certain acidic activation domains. Cell 70(2):251-65
2) Koltovaya NA, et al.  (2003) NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho(-) mutagenesis. Yeast 20(11):955-71
3) Soussi-Boudekou S and Andre B  (1999) A co-activator of nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol Microbiol 31(3):753-62
4) Sterner DE, et al.  (1999) Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol 19(1):86-98
5) Grant PA, et al.  (1997) Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex. Genes Dev 11(13):1640-50
6) Horiuchi J, et al.  (1997) ADA1, a novel component of the ADA/GCN5 complex, has broader effects than GCN5, ADA2, or ADA3. Mol Cell Biol 17(6):3220-8
7) Sterner DE and Berger SL  (2000) Acetylation of histones and transcription-related factors. Microbiol Mol Biol Rev 64(2):435-59
8) Bhaumik SR and Green MR  (2002) Differential requirement of SAGA components for recruitment of TATA-box-binding protein to promoters in vivo. Mol Cell Biol 22(21):7365-71