ACS1/YAL054C Summary Help

Standard Name ACS1 1
Systematic Name YAL054C
Alias FUN44
Feature Type ORF, Verified
Description Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description Acetyl CoA Synthetase 1
Chromosomal Location
ChrI:45022 to 42881 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -63.11 cM
Gene Ontology Annotations All ACS1 GO evidence and references
  View Computational GO annotations for ACS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 13 genes
Classical genetics
Large-scale survey
42 total interaction(s) for 37 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • PCA: 1
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 17
  • Phenotypic Enhancement: 1
  • Positive Genetic: 14
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 713
Molecular Weight (Da) 79,140
Isoelectric Point (pI) 6.6
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrI:45022 to 42881 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: -63.11 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2142 45022..42881 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000000050

ACS1 encodes an acetyl-CoA synthetase (acetate-CoA ligase) (2), which catalyzes the formation of acetyl-CoA from acetate and CoA (6). ACS1 was cloned by its homology to the acetyl-CoA synthetase genes of Aspergillus nidulans and Neurospora crassa (1, 3). An acs1 null mutant can grow with ethanol as the sole carbon source, but there are conflicting reports whether the mutant has a growth defect on acetate medium (1, 3, 7). ACS2 encodes a second acetyl-CoA synthetase (2), and an acs1 acs2 double mutant is inviable (7). ACS1 mRNA levels are reduced when cells are grown on ethanol or glucose (2, 8, 9). ACS1 transcription is positively regulated by Abf1p and negatively regulated by Ume6p (9).

Last updated: 1999-07-30 Contact SGD

References cited on this page View Complete Literature Guide for ACS1
1) De Virgilio C, et al.  (1992) Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast 8(12):1043-51
2) van den Berg MA, et al.  (1996) The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation. J Biol Chem 271(46):28953-9
3) Kratzer S and Schuller HJ  (1995) Carbon source-dependent regulation of the acetyl-coenzyme A synthetase-encoding gene ACS1 from Saccharomyces cerevisiae. Gene 161(1):75-9
4) Guranowski A, et al.  (1994) Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase. J Bacteriol 176(10):2986-90
5) Takahashi H, et al.  (2006) Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription. Mol Cell 23(2):207-17
6) Stryer L  (1988) Biochemistry (3rd ed.). New York: W. H. Freeman and Company
7) Van den Berg MA and Steensma HY  (1995) ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose. Eur J Biochem 231(3):704-13
8) de Jong-Gubbels P, et al.  (1997) The Saccharomyces cerevisiae acetyl-coenzyme A synthetase encoded by the ACS1 gene, but not the ACS2-encoded enzyme, is subject to glucose catabolite inactivation. FEMS Microbiol Lett 153(1):75-81
9) Kratzer S and Schuller HJ  (1997) Transcriptional control of the yeast acetyl-CoA synthetase gene, ACS1, by the positive regulators CAT8 and ADR1 and the pleiotropic repressor UME6. Mol Microbiol 26(4):631-41