ACS1/YAL054C Summary

Standard Name	ACS1 1
Systematic Name	YAL054C
Alias	FUN44
Feature Type	ORF, Verified
Description	Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions (1, 2, 3, 4, 5 and see Summary Paragraph)
Name Description	Acetyl CoA Synthetase 1

Chromosomal Location	ChrI:45022 to 42881 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

	Genetic position: -63.11 cM

Gene Ontology Annotations All ACS1 GO evidence and references

	View Computational GO annotations for ACS1
Molecular Function
Manually curated	acetate-CoA ligase activity (IDA) acid-ammonia (or amide) ligase activity (IDA)
Biological Process
Manually curated	acetate fermentation (IMP) acetyl-CoA biosynthetic process (IDA) histone acetylation (IGI)
Cellular Component
Manually curated	cytosol (IDA)
High-throughput	integral to membrane (ISM) mitochondrion (IDA)

Pathways	acetate utilization ethanol degradation

Mutant phenotypes All ACS1 Phenotype evidence and references

Classical genetics
null	vegetative growth: abnormal
unspecified	resistance to enzymatic treatment: decreased
Large-scale survey
null	competitive fitness: decreased dessication resistance: decreased haploinsufficient resistance to St. John's Wort extract: decreased sporulation efficiency: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased rate
unspecified	resistance to Calcofluor White: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ACS1 Interaction evidence and references

	41 total interaction(s) for 38 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 PCA: 1 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 17 Phenotypic Enhancement: 1 Positive Genetic: 14 Synthetic Lethality: 2 Synthetic Rescue: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ACS1 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrI:45022 to 42881 | |

Note: this feature is encoded on the Crick strand.

: -63.11 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2142	45022..42881	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000000050

SUMMARY PARAGRAPH for ACS1

ACS1 encodes an acetyl-CoA synthetase (acetate-CoA ligase) (2), which catalyzes the formation of acetyl-CoA from acetate and CoA (6). ACS1 was cloned by its homology to the acetyl-CoA synthetase genes of Aspergillus nidulans and Neurospora crassa (1, 3). An acs1 null mutant can grow with ethanol as the sole carbon source, but there are conflicting reports whether the mutant has a growth defect on acetate medium (1, 3, 7). ACS2 encodes a second acetyl-CoA synthetase (2), and an acs1 acs2 double mutant is inviable (7). ACS1 mRNA levels are reduced when cells are grown on ethanol or glucose (2, 8, 9). ACS1 transcription is positively regulated by Abf1p and negatively regulated by Ume6p (9).

Last updated: 1999-07-30

References cited on this page View Complete Literature Guide for ACS1

1)	De Virgilio C, et al. (1992) Cloning and disruption of a gene required for growth on acetate but not on ethanol: the acetyl-coenzyme A synthetase gene of Saccharomyces cerevisiae. Yeast 8(12):1043-51
2)	van den Berg MA, et al. (1996) The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation. J Biol Chem 271(46):28953-9
3)	Kratzer S and Schuller HJ (1995) Carbon source-dependent regulation of the acetyl-coenzyme A synthetase-encoding gene ACS1 from Saccharomyces cerevisiae. Gene 161(1):75-9
4)	Guranowski A, et al. (1994) Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase. J Bacteriol 176(10):2986-90
5)	Takahashi H, et al. (2006) Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription. Mol Cell 23(2):207-17
6)	Stryer L (1988) Biochemistry (3rd ed.). New York: W. H. Freeman and Company
7)	Van den Berg MA and Steensma HY (1995) ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose. Eur J Biochem 231(3):704-13
8)	de Jong-Gubbels P, et al. (1997) The Saccharomyces cerevisiae acetyl-coenzyme A synthetase encoded by the ACS1 gene, but not the ACS2-encoded enzyme, is subject to glucose catabolite inactivation. FEMS Microbiol Lett 153(1):75-81
9)	Kratzer S and Schuller HJ (1997) Transcriptional control of the yeast acetyl-CoA synthetase gene, ACS1, by the positive regulators CAT8 and ADR1 and the pleiotropic repressor UME6. Mol Microbiol 26(4):631-41