ACO1/YLR304C Summary

ACO1 BASIC INFORMATION

Standard Name	ACO1
Systematic Name	YLR304C
Alias	GLU1
Feature Type	ORF, Verified
Description	Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy (1, 2, 3)
Name Description	ACOnitase 1

GO Annotations	All ACO1 GO evidence and references
	View Computational GO annotations for ACO1
Molecular Function
Manually curated	aconitate hydratase activity (TAS) double-stranded DNA binding (IDA) single-stranded DNA binding (IDA)
Biological Process
Manually curated	citrate metabolic process (TAS) glutamate biosynthetic process (TAS) mitochondrial genome maintenance (IMP) propionate metabolic process (IEP) tricarboxylic acid cycle (TAS)
High-throughput	response to drug (IMP)
Cellular Component
Manually curated	cytosol (TAS) mitochondrial matrix (TAS) mitochondrial nucleoid (IDA)
High-throughput	mitochondrion (IDA)

Pathways	glyoxylate cycle superpathway of TCA cycle and glyoxylate cycle TCA cycle, aerobic respiration

Mutant Phenotype	All ACO1 Phenotype details and references
Classical genetics
null	auxotrophy mitochondrial genome maintenance: abnormal Fum1p distribution: abnormal
unspecified	utilization of carbon source: absent
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: decreased fermentative growth: decreased rate hyperosmotic stress resistance: decreased resistance to benomyl: decreased resistance to calcium dichloride: decreased resistance to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO): decreased resistance to ethanol: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent toxin resistance: decreased utilization of carbon source: decreased viable

Interactions	ACO1 All interactions details and references
	36 total interaction(s) for 32 unique genes/features.
Physical Interactions	Affinity Capture-MS: 25 Affinity Capture-RNA: 1 Affinity Capture-Western: 1
Genetic Interactions	Dosage Rescue: 1 Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Synthetic Growth Defect: 4 Synthetic Lethality: 1 Synthetic Rescue: 1

Sequence Information

ChrXII:737550 to 735214 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2004-02-05 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2337	737550..735214	2004-02-05	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004295

ACO1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for ACO1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ACO1]

1)	Gangloff SP, et al. (1990) Molecular cloning of the yeast mitochondrial aconitase gene (ACO1) and evidence of a synergistic regulation of expression by glucose plus glutamate. Mol Cell Biol 10(7):3551-61
2)	Chen XJ, et al. (2005) Aconitase couples metabolic regulation to mitochondrial DNA maintenance. Science 307(5710):714-7
3)	Reinders J, et al. (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906

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