ACN9/YDR511W Summary 
ACN9 BASIC INFORMATION
| Standard Name | ACN9 |
|---|---|
| Systematic Name | YDR511W |
| Feature Type | ORF, Verified |
| Description | Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes (1, 2, 3 and see Summary Paragraph) 
|
| Name Description | ACetate Nonutilizing 1 |
| GO Annotations | All ACN9 GO evidence and references |
|---|---|
| View Computational GO annotations for ACN9 | |
| Molecular Function | |
| Manually curated |
|
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
| Mutant Phenotype | All ACN9 Phenotype details and references |
|---|---|
| Classical genetics | |
| unspecified | |
| Large-scale survey | |
| null |
| Interactions | ACN9 All interactions details and references |
|---|---|
| 1 total interaction(s) for 1 unique genes/features. | |
| Physical Interactions |
|
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
|---|
| Primary SGDID | S000002919 |
|---|
ACN9 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
|
Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for ACN9
SUMMARY PARAGRAPH for ACN9
ACN9 encodes a protein of the mitochondrial intermembrane space that is required for growth on acetate and other nonfermentable carbon sources (1, 3). Mutations in ACN9 lead to elevated levels of enzymes of the glyoxylate cycle, gluconeogenesis, and acetyl-CoA metabolism (1). Although the exact function of Acn9p is unknown, the protein is thought to be involved in gluconeogenesis as acn9 mutants share several phenotypes observed in mutants defective for gluconeogenic enzymes (2).
Gluconeogenesis is the process whereby glucose is synthesized from non-carbohydrate precursors, which enables yeast cells to grow on non-sugar carbon sources like ethanol, glycerol, or peptone. The reactions of gluconeogenesis, shown here, mediate conversion of pyruvate to glucose, which is the opposite of glycolysis, the formation of pyruvate from glucose. While these two pathways have several reactions in common, they are not the exact reverse of each other. As the glycolytic enzymes phosphofructokinase (Pfk1p, Pfk2p) and pyruvate kinase (Cdc19p) only function in the forward direction, the gluconeogenesis pathway replaces those steps with the enzymes pyruvate carboxylase (Pyc1p, Pyc2p) and phosphoenolpyruvate carboxykinase (Pck1p)-generating oxaloacetate as an intermediate from pyruvate to phosphoenolpyruvate-and also the enzyme fructose-1,6-bisphosphatase (Fbp1p) (reviewed in 4). Overall, the gluconeogenic reactions convert two molecules of pyruvate to a molecule of glucose, with the expenditure of six high-energy phosphate bonds, four from ATP and two from GTP.
ACN9 is expressed approximately 100-fold less than tricarboxylic acid and glyoxylate cycle genes and expression is only slightly repressed in the presence of glucose (3). Acn9p homologs have been identified in humans, nematodes, and mice (3).
Last updated: 2005-08-17
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ACN9]
| 1) | McCammon MT (1996) Mutants of Saccharomyces cerevisiae with defects in acetate metabolism: isolation and characterization of Acn- mutants. Genetics 144(1):57-69 |
| 2) | Dennis RA, et al. (1999) Yeast mutants of glucose metabolism with defects in the coordinate regulation of carbon assimilation. Arch Biochem Biophys 365(2):279-88 |
| 3) | Dennis RA and McCammon MT (1999) Acn9 is a novel protein of gluconeogenesis that is located in the mitochondrial intermembrane space. Eur J Biochem 261(1):236-43 |
| 4) | Klein CJ, et al. (1998) Glucose control in Saccharomyces cerevisiae: the role of Mig1 in metabolic functions. Microbiology 144 ( Pt 1):13-24 |
