AAT2/YLR027C Summary Help

Standard Name AAT2 1
Systematic Name YLR027C
Alias ASP5
Feature Type ORF, Verified
Description Cytosolic aspartate aminotransferase involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells (1, 2 and see Summary Paragraph)
Name Description Aspartate AminoTransferase 1
Chromosomal Location
ChrXII:198085 to 196829 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 17 cM
Gene Ontology Annotations All AAT2 GO evidence and references
  View Computational GO annotations for AAT2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
Large-scale survey
186 total interaction(s) for 178 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • PCA: 8
  • Protein-peptide: 5
  • Reconstituted Complex: 3
  • Two-hybrid: 1

Genetic Interactions
  • Negative Genetic: 107
  • Positive Genetic: 23
  • Synthetic Growth Defect: 13
  • Synthetic Lethality: 10
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 418
Molecular Weight (Da) 46,057
Isoelectric Point (pI) 8.5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:198085 to 196829 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 17 cM
Last Update Coordinates: 2011-02-03 | Sequence: 2003-09-22
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1257 198085..196829 2011-02-03 2003-09-22
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004017

AAT2 encodes the aspartate aminotransferase that catalyzes the reversible transfer of the amino group from L-aspartate to 2-oxoglutarate to form oxaloacetate and L-glutamate (2). It plays a role in the metabolism of nitrogen and amino acids, most directly aspartate and glutamate. Aat2p is usually found in the cytoplasm, but is targeted to peroxisomes in cells grown in oleate (1). Aat2p is active in the cell as a homodimer (3). Analysis of the crystal structure of Aat2p reveals that it shares the same three-dimensional fold and active site residues as the aspartate aminotransferases from chicken and E. coli (3). It also shares sequence similarity with the yeast mitochondrial aspartate aminotransferase, Aat1p (4, 2). Yeast strains lacking AAT2 are viable in rich media, but require aspartic acid in minimal media (1). The AAT2 gene was previously known through its mutant form asp5.

Last updated: 1999-09-01 Contact SGD

References cited on this page View Complete Literature Guide for AAT2
1) Verleur N, et al.  (1997) Cytosolic aspartate aminotransferase encoded by the AAT2 gene is targeted to the peroxisomes in oleate-grown Saccharomyces cerevisiae. Eur J Biochem 247(3):972-80
2) Cronin VB, et al.  (1991) The amino acid sequence of the aspartate aminotransferase from baker's yeast (Saccharomyces cerevisiae). Biochem J 277 ( Pt 2)():335-40
3) Jeffery CJ, et al.  (1998) Crystal structure of Saccharomyces cerevisiae cytosolic aspartate aminotransferase. Protein Sci 7(6):1380-7
4) Morin PJ, et al.  (1992) AAT1, a gene encoding a mitochondrial aspartate aminotransferase in Saccharomyces cerevisiae. Biochim Biophys Acta 1171(2):211-4