GPM3/YOL056W Summary Help

GPM3 BASIC INFORMATION

Standard Name GPM3
Systematic Name YOL056W
Feature Type ORF, Verified
Description Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event (1 and see Summary Paragraph)
Name Description Glycerate PhosphoMutase 1
GO Annotations All GPM3 GO evidence and references
    View Computational GO annotations for GPM3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All GPM3 Phenotype details and references
Large-scale survey
null
overexpression
Interactions GPM3 All interactions details and references
7 total interaction(s) for 5 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 1
  • Two-hybrid: 1
Genetic Interactions
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 2
Sequence Information
ChrXV:223267 to 224178 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2006-01-05 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..912 223267..224178 2006-01-05 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000005417

GPM3 RESOURCES

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SGD ORF map
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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for GPM3

SUMMARY PARAGRAPH for GPM3

Gpm2p and Gpm3p were originally identified as homologs of Gpm1p, a phosphoglycerate mutase involved in glycolysis. They share 65% identity with each other and 43% identity with Gpm1p. Gpm2p and Gpm3p each contain a conserved 'LLRHGQSELN' motif, a signature sequence of the phosphoglycerate mutase family. In addition, residues of Gpm1p shown to be involved in catalysis (His8, Arg59, His181) are conserved in Gpm2p and Gpm3p. Despite this homology, neither gene complements a gpm1 deletion in strain VW1A when overexpressed on high copy number plasmids under the control of their weak endogenous promoters. However, GPM2 and GPM3 partially restore activity and growth to a gpm1 deletion mutant when overexpressed from the yeast PFK1 promoter. By following the intermediary metabolites preceding and succeeding the GPM reaction, it has been demonstrated that neither the gpm2 nor the gpm3 deletion mutation affects glycolysis, nor does it confer any obvious phenotype. It has been proposed that GPM2 and GPM3 may be non-functional homologues of GPM1 that were generated by a gene duplication event and then diverged from the parent copy by mutation (2).

Last updated: 2003-09-11

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GPM3]

1) Heinisch JJ, et al.  (1998) Investigation of two yeast genes encoding putative isoenzymes of phosphoglycerate mutase. Yeast 14(3):203-13
2) Rodicio R and Heinisch J  (1987) Isolation of the yeast phosphoglyceromutase gene and construction of deletion mutants. Mol Gen Genet 206(1):133-40