PIG1/YLR273C Summary 
PIG1 BASIC INFORMATION
| Standard Name | PIG1 1 |
|---|---|
| Systematic Name | YLR273C |
| Feature Type | ORF, Verified |
| Description | Putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase (1 and see Summary Paragraph) 
|
| Name Description | Protein Interacting with Gsy2p 1 |
| GO Annotations | All PIG1 GO evidence and references |
|---|---|
| View Computational GO annotations for PIG1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Regulatory Role | |
|---|---|
| Regulatory modules | predicted: stressResponse (643, 547, 373) |
| Mutant Phenotype | All PIG1 Phenotype details and references |
|---|---|
| Classical genetics | |
| null | |
| overexpression | |
| Large-scale survey | |
| null | |
| overexpression |
| Interactions | PIG1 All interactions details and references |
|---|---|
| View additional details at BioGRID | |
| 21 total interaction(s) for 21 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
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| Primary SGDID | S000004263 |
|---|
PIG1 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
|
Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for PIG1
SUMMARY PARAGRAPH for PIG1
Pig1p is a putative targeting subunit for the type-1 protein phosphatase Glc7p that tethers it to the Gsy2p glycogen synthase (1, 2). Pig1p binds Gsy2p in two-hybrid analyses, and PIG1 displays overall similarity to GAC1, suggesting that Pig1p may functionally overlap Gac1p, which also tethers Glc7p to Gsy2p, in glycogen biosynthesis (1).
pig1 gac1 double null mutants display decreased glycogen accumulation compared to either gac1 null mutants or wild type (1). glc7-109 mutants display increased levels of glycogen compared to wild type, and this hyperglycogen phenotype is partially retained in pig1 gac1 gip2 triple null glc7-109 mutants, indicating there may be other targeting subunits capable of targeting Glc7p-109 to Gsy2p (2). The growth of pig1 ppz1 ppz2 triple null mutants appears identical to that of ppz1 ppz2 double null mutants, indicating that there are no genetic interactions between PIG1 and PPZ1 and PPZ2, both of which encode Glc7p-related protein phosphatases (3).
Pig1p, Gac1p, Pig2p and Gip2p are the only four proteins in S. cerevisiae that share a conserved segment of 25 residues, designated the GVNK motif, which is conserved in mammalian type-1 phosphatase targeting subunits (1). Pig1p also has regions of similarity to the Rhizopus glucoamylase precursor AMYG, and also to various mammalian glycogen-associated regulatory subunits of protein phosphatase 1 (1), including rabbit RG RAB and human PPP1R3, mutations in which are associated with non-insulin-dependent diabetes mellitus (4).
Last updated: 2005-10-20
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PIG1]
| 1) | Cheng C, et al. (1997) Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p. Yeast 13(1):1-8 |
| 2) | Williams-Hart T, et al. (2002) Protein phosphatase type 1 regulates ion homeostasis in Saccharomyces cerevisiae. Genetics 160(4):1423-37 |
| 3) | Venturi GM, et al. (2000) Genetic interactions between GLC7, PPZ1 and PPZ2 in saccharomyces cerevisiae. Genetics 155(1):69-83 |
| 4) | Tu J, et al. (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4199-206 |
