OPI3/YJR073C Summary

OPI3 BASIC INFORMATION

Standard Name	OPI3
Systematic Name	YJR073C
Alias	PEM2 1 , 2
Feature Type	ORF, Verified
Description	Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis (1, 3)
Name Description	OverProducer of Inositol 4

GO Annotations	All OPI3 GO evidence and references
	View Computational GO annotations for OPI3
Molecular Function
Manually curated	phosphatidyl-N-methylethanolamine N-methyltransferase activity (TAS)
Biological Process
Manually curated	phosphatidylcholine biosynthetic process (TAS)
Cellular Component
Manually curated	endoplasmic reticulum (TAS)
High-throughput	mitochondrion (IDA)

Pathways	phosphatidic acid and phospholipid biosynthesis phospholipid biosynthesis superpathway of phospholipid biosynthesis

Mutant Phenotype	All OPI3 Phenotype details and references
Classical genetics
null	phospholipids accumulation: abnormal endoplasmic reticulum morphology: abnormal nutrient utilization: decreased resistance to staurosporine: decreased resistance to tunicamycin: decreased resistance to chlorpromazine: decreased toxin resistance: decreased
Large-scale survey
null	glycogen accumulation: increased inositols excretion: increased chitin deposition: normal competitive fitness: decreased filamentous growth: decreased heat sensitivity: increased invasive growth: absent metal resistance: decreased Can1p-GFP distribution: abnormal resistance to Calcofluor White: decreased toxin resistance: decreased viable

Interactions	OPI3 All interactions details and references
	442 total interaction(s) for 256 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Protein-RNA: 1 Reconstituted Complex: 1 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 2 Phenotypic Enhancement: 302 Phenotypic Suppression: 90 Synthetic Growth Defect: 22 Synthetic Lethality: 20

Sequence Information

ChrX:572927 to 572307 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2009-02-18 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..621	572927..572307	2009-02-18	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003834

OPI3 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for OPI3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for OPI3]

1)	Kodaki T and Yamashita S (1987) Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. J Biol Chem 262(32):15428-35
2)	Kodaki T and Yamashita S (1989) Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption. Eur J Biochem 185(2):243-51
3)	McGraw P and Henry SA (1989) Mutations in the Saccharomyces cerevisiae opi3 gene: effects on phospholipid methylation, growth and cross-pathway regulation of inositol synthesis. Genetics 122(2):317-30
4)	Greenberg ML, et al. (1982) Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants. Genetics 100(1):19-33

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