PIG2/YIL045W Summary Help

PIG2 BASIC INFORMATION

Standard Name PIG2 1
Systematic Name YIL045W
Feature Type ORF, Verified
Description Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase (1, 2 and see Summary Paragraph)
Name Description Protein Interacting with Gsy2p 1
GO Annotations All PIG2 GO evidence and references
    View Computational GO annotations for PIG2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Mutant Phenotype All PIG2 Phenotype details and references
Classical genetics
null
Large-scale survey
null
overexpression
Interactions PIG2 All interactions details and references
11 total interaction(s) for 11 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 1
  • Two-hybrid: 5
Genetic Interactions
  • Phenotypic Enhancement: 2
Sequence Information
ChrIX:271160 to 272776 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 1994-12-10 | Sequence: 1994-12-10
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1617 271160..272776 1994-12-10 1994-12-10
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001307

PIG2 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Localization Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for PIG2

SUMMARY PARAGRAPH for PIG2

Pig2p is a putative type-1 protein phosphatase (PP1) targeting subunit that tethers the Glc7p type-1 protein phosphatase to the Gsy2p glycogen synthase (2). Pig2p binds Gsy2p in two-hybrid analyses, and displays 30% similarity to Gip2p, another putative regulatory subunit of the protein phosphatase Glc7p (1).

One traditional analysis has shown that a pig2 null mutant in the EG328-1A strain background accumulates normal levels of glycogen (1). However, in a large-scale study using a homozygous diploid deletion series in the BY4743 strain background, a pig2 null mutant accumulates less glycogen than wild type (3). glc7-109 mutants display increased levels of glycogen, and this hyperglycogen phenotype is partially retained in pig1 gac1 gip2 triple null glc7-109 mutants, indicating that Pig2p may be a redundant targeting subunit capable of targeting Glc7p-109 to Gsy2p (2).

Gac1p, Pig1p, Pig2p and Gip2p are the only four yeast proteins that share a conserved segment of 25 residues, designated the GVNK motif, which is also found in mammalian type-1 protein phosphatase targeting subunits (1). Pig2p also possesses two short regions of similarity to the Rhizopus glucoamylase precursor AMYG, and to various mammalian glycogen-associated regulatory subunits of protein phosphatase 1 (1), including rabbit RG RAB and human PPP1R3, mutations in which are associated with non-insulin-dependent diabetes mellitus (4).

Last updated: 2005-10-25

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PIG2]

1) Cheng C, et al.  (1997) Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p. Yeast 13(1):1-8
2) Williams-Hart T, et al.  (2002) Protein phosphatase type 1 regulates ion homeostasis in Saccharomyces cerevisiae. Genetics 160(4):1423-37
3) Wilson WA, et al.  (2002) Systematic identification of the genes affecting glycogen storage in the yeast Saccharomyces cerevisiae: implication of the vacuole as a determinant of glycogen level. Mol Cell Proteomics 1(3):232-42
4) Tu J, et al.  (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4199-206