SPO11/YHL022C Summary Help

SPO11 BASIC INFORMATION

Standard Name SPO11 1
Systematic Name YHL022C
Feature Type ORF, Verified
Description Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation (2, 3 and see Summary Paragraph)
Name Description SPOrulation
GO Annotations All SPO11 GO evidence and references
    View Computational GO annotations for SPO11
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All SPO11 Phenotype details and references
Classical genetics
conditional
null
reduction of function
Large-scale survey
null
Interactions SPO11 All interactions details and references
61 total interaction(s) for 36 unique genes/features.
Physical Interactions
  • Affinity Capture-Western: 4
  • Biochemical Activity: 2
  • Co-localization: 2
  • Two-hybrid: 8
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 14
  • Phenotypic Suppression: 15
  • Synthetic Lethality: 2
  • Synthetic Rescue: 12
Sequence Information
ChrVIII:64155 to 62959 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -23 cM
Last Update Coordinates: 2005-11-07 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1197 64155..62959 2005-11-07 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000001014

SPO11 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
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  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for SPO11

SUMMARY PARAGRAPH for SPO11

SPO11 encodes a meiosis-specific endonuclease that catalyzes the formation of double-strand breaks (DSBs) that are required to initiate meiotic recombination (4). Spo11p forms a dimer; each monomer breaks one strand of the DNA duplex via a transesterification reaction that forms a covalent intermediate between the catalytic tyrosine (Spo11p-Y135) and the 5' end of the DSB (2, 5). Although Spo11p contains the catalytic residue required for DSB formation, multiple other factors are required for the covalent intermediate to be removed and the DSB to be efficiently processed (6, 7, 8, 9). The Spo11p-DSB intermediate can be isolated in a non-null mutant of RAD50 known as rad50S (2). SPO11 is also required for efficient pairing of homologous chromosomes during meiosis I, but SPO11-independent pathways to chromosome pairing exist as well (10, 11, 12, 13).

Consistent with its central role in initiating meiotic recombination, spo11 mutants do not form DSBs, have decreased levels of recombination, and generate inviable spores due to aneuploidy (14, 15, 16, 11). Formation of DSBs with an exogenous factor, such as ionizing radiation, can partially rescue these phenotypes (17). SPO11 expression during early meiosis is regulated by Ume6p (18). Although Spo11p associated with chromatin throughout the genome, not all regions associated with Spo11p are cleaved to become DSBs (19, 20).

Spo11p contains a Toprim domain, which is found in topoisomerases and primases (21. In particular, Spo11p has similarity to the A subunit of topoisomerase 6 (Top6A), an archaeal type II topoisomerase (22). Spo11p is also conserved in S. pombe (rec12+), C. elegans (spo-11), D. melanogaster (mei-86), and H.sapiens (SPO11) (4 and references therein).

Last updated: 2007-07-20

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for SPO11]

1) Klapholz, S. and Easton-Esposito, R.  (1985) Personal Communication, Mortimer Map Edition 9
2) Keeney S, et al.  (1997) Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell 88(3):375-84
3) Lichten M  (2001) Meiotic recombination: breaking the genome to save it. Curr Biol 11(7):R253-6
4) Keeney S  (2001) Mechanism and control of meiotic recombination initiation. Curr Top Dev Biol 52:1-53
5) Neale MJ, et al.  (2005) Endonucleolytic processing of covalent protein-linked DNA double-strand breaks. Nature 436(7053):1053-7
6) Keeney S and Neale MJ  (2006) Initiation of meiotic recombination by formation of DNA double-strand breaks: mechanism and regulation. Biochem Soc Trans 34(Pt 4):523-5
7) Li J, et al.  (2006) Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation. Genetics 173(4):1969-81
8) Maleki S, et al.  (2007) Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae. Chromosoma 116(5):471-86
9) Sasanuma H, et al.  (2007) Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114. Nucleic Acids Res 35(4):1119-33
10) Anuradha S and Muniyappa K  (2005) Molecular aspects of meiotic chromosome synapsis and recombination. Prog Nucleic Acid Res Mol Biol 79:49-132
11) Weiner BM and Kleckner N  (1994) Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast. Cell 77(7):977-91
12) Bhuiyan H and Schmekel K  (2004) Meiotic chromosome synapsis in yeast can occur without spo11-induced DNA double-strand breaks. Genetics 168(2):775-83
13) Peoples-Holst TL and Burgess SM  (2005) Multiple branches of the meiotic recombination pathway contribute independently to homolog pairing and stable juxtaposition during meiosis in budding yeast. Genes Dev 19(7):863-74
14) Cao L, et al.  (1990) A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae. Cell 61(6):1089-101
15) Klapholz S, et al.  (1985) The role of the SPO11 gene in meiotic recombination in yeast. Genetics 110(2):187-216
16) Wagstaff JE, et al.  (1985) Meiotic exchange within and between chromosomes requires a common Rec function in Saccharomyces cerevisiae. Mol Cell Biol 5(12):3532-44
17) Thorne LW and Byers B  (1993) Stage-specific effects of X-irradiation on yeast meiosis. Genetics 134(1):29-42
18) Steber CM and Esposito RE  (1995) UME6 is a central component of a developmental regulatory switch controlling meiosis-specific gene expression. Proc Natl Acad Sci U S A 92(26):12490-4
19) Prieler S, et al.  (2005) The control of Spo11's interaction with meiotic recombination hotspots. Genes Dev 19(2):255-69
20) Robine N, et al.  (2007) Genome-Wide Redistribution of Meiotic Double-Strand Breaks in Saccharomyces cerevisiae. Mol Cell Biol 27(5):1868-80
21) Diaz RL, et al.  (2002) Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation. Mol Cell Biol 22(4):1106-15
22) Bergerat A, et al.  (1997) An atypical topoisomerase II from Archaea with implications for meiotic recombination. Nature 386(6623):414-7