GIP2/YER054C Summary 
GIP2 BASIC INFORMATION
| Standard Name | GIP2 1 |
|---|---|
| Systematic Name | YER054C |
| Feature Type | ORF, Verified |
| Description | Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p (1, 2 and see Summary Paragraph) 
|
| Name Description | Glc7-Interacting Protein 1 |
| GO Annotations | All GIP2 GO evidence and references |
|---|---|
| View Computational GO annotations for GIP2 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated |
| Mutant Phenotype | All GIP2 Phenotype details and references |
|---|---|
| Classical genetics | |
| null | |
| Large-scale survey | |
| null | |
| overexpression |
| Interactions | GIP2 All interactions details and references |
|---|---|
| 15 total interaction(s) for 11 unique genes/features. | |
| Physical Interactions |
|
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB |
|---|
| Primary SGDID | S000000856 |
|---|
GIP2 RESOURCES
| SGD ORF map | GBrowse |
|---|---|
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Click on histogram for expression summary
Expression Summary
ADDITIONAL INFORMATION for GIP2
SUMMARY PARAGRAPH for GIP2
Gip2p is a nonessential protein involved in glycogen accumulation, and may be a regulatory subunit of the Glc7p type-1 protein phosphatase (PP1) (3, 2). Gip2p displays similarity to Gac1p, which is a regulatory subunit for Glc7p, and also to Pig1p and Pig2p, which bind the Gsy2p glycogen synthase in two-hybrid assays (3, 1, 2). Indeed, Gip2p, Gac1p, Pig1p, and Pig2p are the only four proteins in the yeast genome that share a conserved segment of approximately 25 residues, designated the GVNK motif, that is identifiable also in RGI, the mammalian type 1 phosphatase targeting subunit (3). Two-hybrid analyses indicate that Gip2p does in fact bind Glc7p, as well as a truncated species of the Ppz1p protein phosphatase (Ppz1p-[361-692]) (1, 4).
GIP2 is induced in response to ethanol shock (5). GIP2 induction has also been demonstrated in three of three optimally adapted strains from independent cultures after continuous aerobic growth for more than 250 generations in glucose-limited chemostats, suggesting that increased expression of GIP2 is associated with increased fitness during conditions of limited glucose (6). Although Gip2 null mutants are viable (3), they do accumulate decreased levels of glycogen. Also, a gip2 null glc7-109 double mutant accumulates less glycogen than a glc7-109 mutant alone (1).
Gip2p possesses two short regions of similarity to the Rhizopus glucoamylase precursor AMYG, and also to various mammalian glycogen-associated regulatory subunits of protein phosphatase 1 (3), including rabbit RG RAB and human PPP1R3, mutations in which are associated with non-insulin-dependent diabetes mellitus (1).
Last updated: 2005-10-13
REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GIP2]
| 1) | Tu J, et al. (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol Cell Biol 16(8):4199-206 |
| 2) | Williams-Hart T, et al. (2002) Protein phosphatase type 1 regulates ion homeostasis in Saccharomyces cerevisiae. Genetics 160(4):1423-37 |
| 3) | Cheng C, et al. (1997) Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that interacts with the yeast glycogen synthase Gsy2p. Yeast 13(1):1-8 |
| 4) | Venturi GM, et al. (2000) Genetic interactions between GLC7, PPZ1 and PPZ2 in saccharomyces cerevisiae. Genetics 155(1):69-83 |
| 5) | Alexandre H, et al. (2001) Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae. FEBS Lett 498(1):98-103 |
| 6) | Ferea TL, et al. (1999) Systematic changes in gene expression patterns following adaptive evolution in yeast. Proc Natl Acad Sci U S A 96(17):9721-6 |
