HEM14/YER014W Summary

HEM14 BASIC INFORMATION

Standard Name	HEM14 1
Systematic Name	YER014W
Feature Type	ORF, Verified
Description	Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides (1, 2, 3)
Name Description	HEMe biosynthesis 1

GO Annotations	All HEM14 GO evidence and references
	View Computational GO annotations for HEM14
Molecular Function
Manually curated	oxygen-dependent protoporphyrinogen oxidase activity (IDA, IMP)
Biological Process
Manually curated	heme biosynthetic process (IMP)
Cellular Component
Manually curated	mitochondrial inner membrane (IDA)
High-throughput	mitochondrion (IDA)

Pathways	heme biosynthesis superpathway of heme and siroheme biosynthesis

Mutant Phenotype	All HEM14 Phenotype details and references
Classical genetics
null	resistance to sampangine: decreased
unspecified	heme accumulation: absent utilization of carbon source: absent
Large-scale survey
null	budding pattern: abnormal cell shape: abnormal competitive fitness: decreased heat sensitivity: increased lipid particle morphology: abnormal resistance to sulfanilamide: decreased resistance to doxorubicin: decreased respiratory growth: decreased rate respiratory growth: absent viable

Interactions	HEM14 All interactions details and references
	View additional details at BioGRID
	21 total interaction(s) for 21 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Two-hybrid: 1
Genetic Interactions	Synthetic Growth Defect: 15 Synthetic Lethality: 4

Sequence Information

ChrV:182599 to 184218 | |

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1620	182599..184218	1996-07-31	1996-07-31

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000816

HEM14 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for HEM14

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for HEM14]

1)	Urban-Grimal D and Labbe-Bois R (1981) Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Mol Gen Genet 183(1):85-92
2)	Glerum DM, et al. (1996) Cloning and identification of HEM14, the yeast gene for mitochondrial protoporphyrinogen oxidase. Yeast 12(14):1421-5
3)	Camadro JM and Labbe P (1996) Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides. J Biol Chem 271(15):9120-8

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