PMI40/YER003C Summary Help

PMI40 BASIC INFORMATION

Standard Name PMI40 1
Systematic Name YER003C
Feature Type ORF, Verified
Description Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation (1 and see Summary Paragraph)
Also known as: PMI
Name Description PhosphoMannose Isomerase 1
GO Annotations All PMI40 GO evidence and references
    View Computational GO annotations for PMI40
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Pathways
Mutant Phenotype All PMI40 Phenotype details and references
Large-scale survey
conditional
null
reduction of function
Interactions PMI40 All interactions details and references
  View additional details at BioGRID
12 total interaction(s) for 12 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 1
  • Two-hybrid: 1
Genetic Interactions
  • Synthetic Growth Defect: 5
Sequence Information
ChrV:159117 to 157735 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..31 159117..159087 1996-07-31 1996-07-31
Intron 32..124 159086..158994 1996-07-31 1996-07-31
CDS 125..1383 158993..157735 1996-07-31 1996-07-31
Post-translational Modifications PhosphoGRID | PhosphoPep Database
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000000805

PMI40 RESOURCES

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SGD ORF map
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  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for PMI40

SUMMARY PARAGRAPH for PMI40

PMI40 encodes phosphomannose isomerase (EC 5.3.1.8), which catalyzes the isomerization between mannose-6-phosphate (M6P) and fructose-6-phosphate; M6P is a substrate for Sec53p and is used as a source of mannose for N-linked and O-linked glycosylation of proteins and GPI anchoring. Deletion or heat-inactivation of Pmi40p results in mannose auxotrophy, clumping, abnormal morphology, and defective secretion and cell-surface presentation of glycoproteins (2); unlike sec53, the pmi40 phenotype is suppressed if the growth medium is supplemented with mannose, presumably since mannose is imported into the cell as M6P, bypassing the need for PMI40 (1). Pmi40p is a zinc-dependent metalloenzyme; zinc, cadmium, and mercury are competitive inhibitors (3).

Mutations in the human homolog, PMI1 (OMIM), cause the congenital disorder of glycosylation CDG-Ib (OMIM). The human (4), Candida albicans (5), and Hansenula polymorpha (6) homologs all complement the pmi40 mutation. Deletion of the homolog MAN1 disrupts the pathogenicity of Cryptococcus neoformans (7).

Last updated: 2005-07-01

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PMI40]

1) Smith DJ, et al.  (1992) PMI40, an intron-containing gene required for early steps in yeast mannosylation. Mol Cell Biol 12(7):2924-30
2) Payton MA, et al.  (1991) A novel Saccharomyces cerevisiae secretory mutant possesses a thermolabile phosphomannose isomerase. J Bacteriol 173(6):2006-10
3) Wells TN, et al.  (1993) Phosphomannose isomerase from Saccharomyces cerevisiae contains two inhibitory metal ion binding sites. Biochemistry 32(5):1294-301
4) Proudfoot AE, et al.  (1994) Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase. Eur J Biochem 219(1-2):415-23
5) Smith DJ, et al.  (1995) Cloning and heterologous expression of the Candida albicans gene PMI 1 encoding phosphomannose isomerase. Yeast 11(4):301-10
6) Kim MW, et al.  (2002) Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes. Yeast 19(10):863-71
7) Wills EA, et al.  (2001) Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity. Mol Microbiol 40(3):610-20