UBC8/YEL012W Summary

UBC8 BASIC INFORMATION

Standard Name	UBC8 1
Systematic Name	YEL012W
Alias	GID3 2
Feature Type	ORF, Verified
Description	Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro (1, 3, 4)
Name Description	UBiquitin-Conjugating 1

GO Annotations	All UBC8 GO evidence and references
	View Computational GO annotations for UBC8
Molecular Function
Manually curated	ubiquitin-protein ligase activity (IDA, ISS)
Biological Process
Manually curated	negative regulation of gluconeogenesis (IMP) proteasomal ubiquitin-dependent protein catabolic process (IMP) protein monoubiquitination (TAS) protein polyubiquitination (TAS) protein ubiquitination involved in ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
Manually curated	cytoplasm (IDA)

Mutant Phenotype	All UBC8 Phenotype details and references
Classical genetics
null	Myc9-Gid4p accumulation: increased Fbp1p accumulation: increased resistance to rapamycin: decreased
Large-scale survey
null	glutathione excretion: increased competitive fitness: decreased heat sensitivity: increased resistance to arsenite(3-): decreased respiratory growth: decreased toxin resistance: decreased viable

Interactions	UBC8 All interactions details and references
	View additional details at BioGRID
	63 total interaction(s) for 63 unique genes/features.
Physical Interactions	Affinity Capture-MS: 2 Affinity Capture-RNA: 1 Biochemical Activity: 1 Two-hybrid: 1
Genetic Interactions	Negative Genetic: 52 Positive Genetic: 5 Synthetic Rescue: 1

Sequence Information

ChrV:131772 to 132551 | |

Last Update

Coordinates: 2000-12-01 | Sequence: 2000-12-01

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..5	131772..131776	2000-12-01	2000-12-01
Intron	6..128	131777..131899	2000-12-01	2000-12-01
CDS	129..780	131900..132551	2000-12-01	2000-12-01

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000000738

UBC8 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for UBC8

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for UBC8]

1)	Qin S, et al. (1991) Cloning and characterization of a Saccharomyces cerevisiae gene encoding a new member of the ubiquitin-conjugating protein family. J Biol Chem 266(23):15549-54
2)	Schule T, et al. (2000) Ubc8p functions in catabolite degradation of fructose-1, 6-bisphosphatase in yeast. EMBO J 19(10):2161-7
3)	Kaiser P, et al. (1994) A human ubiquitin-conjugating enzyme homologous to yeast UBC8. J Biol Chem 269(12):8797-802
4)	Regelmann J, et al. (2003) Catabolite degradation of fructose-1,6-bisphosphatase in the yeast Saccharomyces cerevisiae: a genome-wide screen identifies eight novel GID genes and indicates the existence of two degradation pathways. Mol Biol Cell 14(4):1652-63

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