MAK3/YPR051W Summary Help

Standard Name MAK3 1
Systematic Name YPR051W
Alias NAA30 2
Feature Type ORF, Verified
Description Catalytic subunit of the NatC type N-terminal acetyltransferase; involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus (3, 4, 5, 6, 7)
Name Description MAintenance of Killer 8
Chromosomal Location
ChrXVI:664960 to 665490 | ORF Map | GBrowse
Genetic position: 34 cM
Gene Ontology Annotations All MAK3 GO evidence and references
  View Computational GO annotations for MAK3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Classical genetics
Large-scale survey
84 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 2
  • Biochemical Activity: 1
  • Co-fractionation: 2
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 39
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 5
  • Synthetic Growth Defect: 17
  • Synthetic Lethality: 2
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 176
Molecular Weight (Da) 20,457
Isoelectric Point (pI) 7.37
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXVI:664960 to 665490 | ORF Map | GBrowse
Genetic position: 34 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..531 664960..665490 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000006255
References cited on this page View Complete Literature Guide for MAK3
1) Wickner RB and Toh-e A  (1982) [HOK], a new yeast non-Mendelian trait, enables a replication-defective killer plasmid to be maintained. Genetics 100(2):159-74
2) Polevoda B, et al.  (2009) A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates. BMC Proc 3 Suppl 6:S2
3) Wickner RB and Leibowitz MJ  (1976) Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast. J Mol Biol 105(3):427-43
4) Polevoda B and Sherman F  (2001) NatC Nalpha-terminal acetyltransferase of yeast contains three subunits, Mak3p, Mak10p, and Mak31p. J Biol Chem 276(23):20154-9
5) Behnia R, et al.  (2004) Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p. Nat Cell Biol 6(5):405-13
6) Setty SR, et al.  (2004) Golgi targeting of ARF-like GTPase Arl3p requires its Nalpha-acetylation and the integral membrane protein Sys1p. Nat Cell Biol 6(5):414-9
7) Behnia R, et al.  (2007) The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic. J Cell Biol 176(3):255-61
8) Wickner RB  (1974) Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics 76(3):423-32