GAL4/YPL248C Summary Help

GAL4 BASIC INFORMATION

Standard Name GAL4 1, 2
Systematic Name YPL248C
Alias GAL81 3
Feature Type ORF, Verified
Description DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p (4 and see Summary Paragraph)
Name Description GALactose metabolism
GO Annotations All GAL4 GO evidence and references
    View Computational GO annotations for GAL4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulatory Role
Binding motifs Predicted GAL4 Binding Site Locations
    Regulatory modules predicted: stressResponse (457)
    Mutant Phenotype All GAL4 Phenotype details and references
    Classical genetics
    conditional
    null
    reduction of function
    unspecified
    Large-scale survey
    null
    Interactions GAL4 All interactions details and references
      View additional details at BioGRID
    171 total interaction(s) for 78 unique genes/features.
    Physical Interactions
    • Affinity Capture-RNA: 2
    • Affinity Capture-Western: 34
    • Biochemical Activity: 3
    • Co-crystal Structure: 3
    • Co-localization: 14
    • Co-purification: 1
    • Far Western: 1
    • FRET: 1
    • PCA: 31
    • Protein-peptide: 1
    • Reconstituted Complex: 46
    • Two-hybrid: 12
    Genetic Interactions
    • Dosage Lethality: 1
    • Dosage Rescue: 2
    • Negative Genetic: 11
    • Phenotypic Enhancement: 1
    • Phenotypic Suppression: 4
    • Positive Genetic: 3
    Sequence Information
    ChrXVI:82356 to 79711 | ORF Map | GBrowse
    Note: this feature is encoded on the Crick strand.
    Gbrowse
    Genetic position: -143 cM
    Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
    Subfeature details
    Relative
    Coordinates    		 Chromosomal
    Coordinates    		 Most Recent Updates
    Coordinates Sequence
    CDS 1..2646 82356..79711 1996-07-31 1996-07-31
    Post-translational Modifications PhosphoGRID | PhosphoPep Database
    External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
    Primary SGDIDS000006169

    GAL4 RESOURCES

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    SGD ORF mapGBrowse
    SGD ORF map
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    • Literature
    • Retrieve Sequences
    • Sequence Analysis Tools
    • Protein Info & Structure
    • Localization Resources
    • Interactions
    • Phenotype Resources
    • Maps & Displays
    • Comparison Resources
    • Functional Analysis

    Click on histogram for expression summary
    Expression Summary histogram

    ADDITIONAL INFORMATION for GAL4

    SUMMARY PARAGRAPH for GAL4

    GAL4 encodes one of the best studied transcription factors in the explored universe (5, 6, 7). In the presence of galactose, Gal4p activates transcription of the GAL structural genes, which encode galactose metabolic proteins. Gal4p binds to a specific sequence, the UASG, in the promoters of GAL structural genes. The transcriptional regulation of yeast GAL genes has been thoroughly reviewed in reference 7.

    Among the promoters regulated by Gal4p is the one shared by the divergently transcribed genes GAL1 (encoding galactokinase) and GAL10 (an epimerase) (8, 7). The GAL1-GAL10 promoter has been widely used to conditionally overexpress genes in yeast (for example, see reference 9).

    The DNA binding domain of Gal4p can be physically separated from its transcription activation domain (7). Many versions of the yeast two-hybrid, or interaction trap, method, use the Gal4p DNA binding and transcription activation domains (reviewed in reference 10).

    Last updated: 1999-12-20

    REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for GAL4]

    1) Haber, J.  (1985) Personal Communication, Mortimer Map Edition 9
    2) Link, A. and Olson, M.  (1989) Personal Communication, Mortimer Map Edition 10
    3) Hopper JE and Rowe LB  (1978) Molecular expression and regulation of the galactose pathway genes in Saccharomyces cerevisiae. Distinct messenger RNAs specified by the Gali and Gal7 genes in the Gal7-Gal10-Gal1 cluster. J Biol Chem 253(20):7566-9
    4) Bhat PJ and Murthy TV  (2001) Transcriptional control of the GAL/MEL regulon of yeast Saccharomyces cerevisiae: mechanism of galactose-mediated signal transduction. Mol Microbiol 40(5):1059-66
    5) Hashimoto H, et al.  (1983) Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae. Isolation and characterization of the regulatory gene GAL4. Mol Gen Genet 191(1):31-8
    6) Keegan L, et al.  (1986) Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science 231(4739):699-704
    7) Lohr D, et al.  (1995) Transcriptional regulation in the yeast GAL gene family: a complex genetic network. FASEB J 9(9):777-87
    8) Fedor MJ and Kornberg RD  (1989) Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1-GAL10 genes. Mol Cell Biol 9(4):1721-32
    9) Schneider JC and Guarente L  (1991) Vectors for expression of cloned genes in yeast: regulation, overproduction, and underproduction. Methods Enzymol 194:373-88
    10) Brent R and Finley RL Jr  (1997) Understanding gene and allele function with two-hybrid methods. Annu Rev Genet 31:663-704
    11) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
    12) Zhu J and Zhang MQ  (1999) SCPD: a promoter database of the yeast Saccharomyces cerevisiae. Bioinformatics 15(7-8):607-11
    13) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66