CDC73/YLR418C Summary Help

Standard Name CDC73 1
Systematic Name YLR418C
Feature Type ORF, Verified
Description Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homologue, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers (2, 3, 4, 5, 6, 7, 8, 9, 10)
Name Description Cell Division Cycle 1
Chromosomal Location
ChrXII:958095 to 956914 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All CDC73 GO evidence and references
  View Computational GO annotations for CDC73
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 92 genes
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
1192 total interaction(s) for 675 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 117
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 28
  • Co-fractionation: 1
  • Co-purification: 4
  • PCA: 8
  • Protein-RNA: 4
  • Reconstituted Complex: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 376
  • Phenotypic Suppression: 1
  • Positive Genetic: 57
  • Synthetic Growth Defect: 196
  • Synthetic Lethality: 390
  • Synthetic Rescue: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 393
Molecular Weight (Da) 44,456
Isoelectric Point (pI) 8.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:958095 to 956914 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1182 958095..956914 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004410
References cited on this page View Complete Literature Guide for CDC73
1) Reed SI, et al.  (1988) Isolation and characterization of two genes encoding yeast mating pheromone signaling elements: CDC72 and CDC73. Cold Spring Harb Symp Quant Biol 53 Pt 2:621-7
2) Chang M, et al.  (1999) A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling. Mol Cell Biol 19(2):1056-67
3) Shi X, et al.  (1997) Cdc73p and Paf1p are found in a novel RNA polymerase II-containing complex distinct from the Srbp-containing holoenzyme. Mol Cell Biol 17(3):1160-9
4) Mueller CL and Jaehning JA  (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
5) Krogan NJ, et al.  (2003) The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Mol Cell 11(3):721-9
6) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
7) Zhang Y, et al.  (2009) The Paf1 complex is required for efficient transcription elongation by RNA polymerase I. Proc Natl Acad Sci U S A 106(7):2153-8
8) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
9) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
10) Chen H, et al.  (2012) Crystallographic analysis of the conserved C-terminal domain of transcription factor Cdc73 from Saccharomyces cerevisiae reveals a GTPase-like fold. Acta Crystallogr D Biol Crystallogr 68(Pt 8):953-9