MEC3/YLR288C Summary Help

Standard Name MEC3 1
Systematic Name YLR288C
Alias PIP3 , PSO9 2
Feature Type ORF, Verified
Description DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 (3, 4, 5, 6, 7)
Name Description Mitosis Entry Checkpoint 1
Chromosomal Location
ChrXII:714904 to 713480 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MEC3 GO evidence and references
  View Computational GO annotations for MEC3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
Large-scale survey
180 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-Western: 13
  • Co-fractionation: 2
  • Co-purification: 1
  • PCA: 2
  • Reconstituted Complex: 3
  • Two-hybrid: 22

Genetic Interactions
  • Dosage Growth Defect: 3
  • Dosage Rescue: 2
  • Negative Genetic: 29
  • Phenotypic Enhancement: 23
  • Phenotypic Suppression: 1
  • Positive Genetic: 1
  • Synthetic Growth Defect: 42
  • Synthetic Lethality: 14
  • Synthetic Rescue: 14

Expression Summary
Length (a.a.) 474
Molecular Weight (Da) 53,145
Isoelectric Point (pI) 5.1
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:714904 to 713480 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1425 714904..713480 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004279
References cited on this page View Complete Literature Guide for MEC3
1) Weinert TA, et al.  (1994) Mitotic checkpoint genes in budding yeast and the dependence of mitosis on DNA replication and repair. Genes Dev 8(6):652-65
2) Cardone JM, et al.  (2006) Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. DNA Repair (Amst) 5(2):163-71
3) Kondo T, et al.  (1999) Role of a complex containing Rad17, Mec3, and Ddc1 in the yeast DNA damage checkpoint pathway. Mol Cell Biol 19(2):1136-43
4) de la Torre-Ruiz M and Lowndes NF  (2000) The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining. FEBS Lett 467(2-3):311-5
5) Hong EJ and Roeder GS  (2002) A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev 16(3):363-76
6) Majka J and Burgers PM  (2003) Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint. Proc Natl Acad Sci U S A 100(5):2249-54
7) Rouse J and Jackson SP  (2002) Interfaces between the detection, signaling, and repair of DNA damage. Science 297(5581):547-51