NUP85/YJR042W Summary Help

Standard Name NUP85 1
Systematic Name YJR042W
Alias RAT9 2
Feature Type ORF, Verified
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 (1, 3, 4, 5, 6, 7, 8, 9, 10 and see Summary Paragraph)
Name Description NUclear Pore 1
Chromosomal Location
ChrX:514055 to 516289 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NUP85 GO evidence and references
  View Computational GO annotations for NUP85
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 4 genes
Resources
Classical genetics
conditional
Large-scale survey
conditional
null
repressible
Resources
131 total interaction(s) for 38 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 65
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 8
  • FRET: 3
  • PCA: 8
  • Reconstituted Complex: 10
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 10
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 744
Molecular Weight (Da) 84,897
Isoelectric Point (pI) 4.33
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:514055 to 516289 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2235 514055..516289 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003803
SUMMARY PARAGRAPH for NUP85

NUP85 encodes a nuclear pore protein that was identified independently by screens for mutants defective in mRNA export from the nucleus (2) and for synthetic lethals with an nsp1 temperature sensitive mutation (11, 12). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 12, 13, 14, 15). The structure of the vertebrate NPC has been studied extensively; recent reviews include 16, 17, 18, and 19. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (20, 21). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 12, 13, 22, 11, 23). Nup85p is part of subcomplex within the NPC that also includes Sec13p, Seh1p, Nup84p, and Nup120p (1, 12). Nup85p is tightly associated with Seh1p in the subcomplex (1). While Nup85p is not essential, nup85 deletion strains are temperature sensitive and show defects in nuclear envelope morphology and NPC distribution (2, 1). The nup85 deletion is synthetically lethal with mutations in the mRNA export factors Mex67p and Mtr2p (24, 25), and Mtr2p interacts physically with Nup85p (25).

Last updated: 1999-08-04 Contact SGD

References cited on this page View Complete Literature Guide for NUP85
1) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
2) Goldstein AL, et al.  (1996) Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol Biol Cell 7(6):917-34
3) Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54
4) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
5) Gao H, et al.  (2003) Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem 278(28):25331-40
6) Menon BB, et al.  (2005) Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation. Proc Natl Acad Sci U S A 102(16):5749-54
7) Lutzmann M, et al.  (2002) Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J 21(3):387-97
8) Fernandez-Martinez J and Rout MP  (2009) Nuclear pore complex biogenesis. Curr Opin Cell Biol 21(4):603-12
9) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64
10) Fernandez-Martinez J, et al.  (2012) Structure-function mapping of a heptameric module in the nuclear pore complex. J Cell Biol 196(4):419-34
11) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
12) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
13) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
14) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
15) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
16) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
17) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
18) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
19) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
20) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
21) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
22) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
23) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
24) Segref A, et al.  (1997) Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J 16(11):3256-71
25) Santos-Rosa H, et al.  (1998) Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol Cell Biol 18(11):6826-38