BUB1/YGR188C Summary Help

Standard Name BUB1 1
Systematic Name YGR188C
Feature Type ORF, Verified
Description Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication (2, 3, 4, 5, 6, 7, 8 and see Summary Paragraph)
Name Description Budding Uninhibited by Benzimidazole 1
Chromosomal Location
ChrVII:875109 to 872044 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 150 cM
Gene Ontology Annotations All BUB1 GO evidence and references
  View Computational GO annotations for BUB1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 6 genes
Classical genetics
reduction of function
Large-scale survey
679 total interaction(s) for 473 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 10
  • Biochemical Activity: 2
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 14

Genetic Interactions
  • Dosage Lethality: 2
  • Dosage Rescue: 6
  • Negative Genetic: 310
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 6
  • Positive Genetic: 87
  • Synthetic Growth Defect: 21
  • Synthetic Lethality: 196
  • Synthetic Rescue: 6

Expression Summary
Length (a.a.) 1,021
Molecular Weight (Da) 117,867
Isoelectric Point (pI) 6.69
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:875109 to 872044 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Genetic position: 150 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3066 875109..872044 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003420

BUB1 encodes a non-essential protein kinase involved in the spindle checkpoint (3). The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules (see 9 for review; reference 10 describes defects detected by the checkpoint). The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators (9). Bub1p binds to and phosphorylates another spindle checkpoint protein, Bub3p, and autophosphorylates (3). Like other spindle checkpoint mutants, bub1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization (1). Overexpression of a dominant mutant, BUB1-5, delays mitosis, as does overexpression of MPS1, which encodes another protein kinase involved in the spindle checkpoint (2, 11). The BUB1-5 mitotic delay requires other spindle checkpoint components including Mps1p, Bub2p, Bub3p, Mad1p, Mad2p, and Mad3p (2). Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation (12, 13, 14, 15). Mad3p has sequence similarity to Bub1p, but the similarity does not include the kinase domain of Bub1p (16). Protein kinases related to Bub1p are involved in checkpoint control and timing of mitosis in several other species, including S. pombe (17), mouse (18), and human (19). Mutations in the human BUB1 homologs (BUB1, BUB1B) are associated with several types of cancer (20, 21).

Last updated: 2005-07-01 Contact SGD

References cited on this page View Complete Literature Guide for BUB1
1) Hoyt MA, et al.  (1991) S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66(3):507-17
2) Farr KA and Hoyt MA  (1998) Bub1p kinase activates the Saccharomyces cerevisiae spindle assembly checkpoint. Mol Cell Biol 18(5):2738-47
3) Roberts BT, et al.  (1994) The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol Cell Biol 14(12):8282-91
4) Brady DM and Hardwick KG  (2000) Complex formation between Mad1p, Bub1p and Bub3p is crucial for spindle checkpoint function. Curr Biol 10(11):675-8
5) Kitagawa K, et al.  (2003) Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Mol Cell 11(5):1201-13
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Goto GH, et al.  (2011) Bub1-mediated adaptation of the spindle checkpoint. PLoS Genet 7(1):e1001282
8) Nerusheva OO, et al.  (2014) Tension-dependent removal of pericentromeric shugoshin is an indicator of sister chromosome biorientation. Genes Dev 28(12):1291-309
9) Hardwick KG  (1998) The spindle checkpoint. Trends Genet 14(1):1-4
10) Hardwick KG, et al.  (1999) Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics 152(2):509-18
11) Hardwick KG, et al.  (1996) Activation of the budding yeast spindle assembly checkpoint without mitotic spindle disruption. Science 273(5277):953-6
12) Alexandru G, et al.  (1999) Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J 18(10):2707-21
13) Fesquet D, et al.  (1999) A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J 18(9):2424-34
14) Fraschini R, et al.  (1999) Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol 145(5):979-91
15) Li R  (1999) Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc Natl Acad Sci U S A 96(9):4989-94
16) Hardwick KG, et al.  (2000) MAD3 encodes a novel component of the spindle checkpoint which interacts with Bub3p, Cdc20p, and Mad2p. J Cell Biol 148(5):871-82
17) Bernard P, et al.  (1998) Fission yeast bub1 is a mitotic centromere protein essential for the spindle checkpoint and the preservation of correct ploidy through mitosis. J Cell Biol 143(7):1775-87
18) Taylor SS and McKeon F  (1997) Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage. Cell 89(5):727-35
19) Ouyang B, et al.  (1998) Human Bub1: a putative spindle checkpoint kinase closely linked to cell proliferation. Cell Growth Differ 9(10):877-85
20) Cahill DP, et al.  (1998) Mutations of mitotic checkpoint genes in human cancers. Nature 392(6673):300-3
21) Yamaguchi K, et al.  (1999) Mutation analysis of hBUB1 in aneuploid HNSCC and lung cancer cell lines. Cancer Lett 139(2):183-7