SRB5/YGR104C Summary Help

Standard Name SRB5 1
Systematic Name YGR104C
Alias MED18 2
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance (1, 3, 4, 5, 6)
Name Description Suppressor of RNA polymerase B 1
Chromosomal Location
ChrVII:698368 to 697445 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SRB5 GO evidence and references
  View Computational GO annotations for SRB5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 158 genes
Regulators 3 genes
Classical genetics
Large-scale survey
329 total interaction(s) for 90 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 142
  • Affinity Capture-Western: 103
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 36
  • PCA: 2
  • Reconstituted Complex: 11
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Rescue: 2
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 17
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 307
Molecular Weight (Da) 34,288
Isoelectric Point (pI) 4.69
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:698368 to 697445 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..924 698368..697445 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003336
References cited on this page View Complete Literature Guide for SRB5
1) Thompson CM, et al.  (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Kim YJ, et al.  (1994) A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77(4):599-608
4) Kornberg RD  (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
5) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
6) Mukundan B and Ansari A  (2011) Novel role for mediator complex subunit Srb5/Med18 in termination of transcription. J Biol Chem 286(43):37053-7