RTF1/YGL244W Summary Help

Standard Name RTF1 1
Systematic Name YGL244W
Alias CSL3 2
Feature Type ORF, Verified
Description Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay (1, 3, 4, 5, 6)
Name Description Restores TBP Function 1
Chromosomal Location
ChrVII:41498 to 43174 | ORF Map | GBrowse
Gene Ontology Annotations All RTF1 GO evidence and references
  View Computational GO annotations for RTF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 454 genes
Regulators 116 genes
Classical genetics
Large-scale survey
582 total interaction(s) for 322 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 81
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 21
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 1
  • Protein-RNA: 4
  • Reconstituted Complex: 3
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 6
  • Negative Genetic: 260
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 1
  • Positive Genetic: 63
  • Synthetic Growth Defect: 57
  • Synthetic Lethality: 58
  • Synthetic Rescue: 14

Expression Summary
Length (a.a.) 558
Molecular Weight (Da) 65,868
Isoelectric Point (pI) 5.12
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:41498 to 43174 | ORF Map | GBrowse
Last Update Coordinates: 1999-07-17 | Sequence: 1999-07-17
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1677 41498..43174 1999-07-17 1999-07-17
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003213
References cited on this page View Complete Literature Guide for RTF1
1) Stolinski LA, et al.  (1997) Identification of RTF1, a novel gene important for TATA site selection by TATA box-binding protein in Saccharomyces cerevisiae. Mol Cell Biol 17(8):4490-500
2) Baker RE, et al.  (1998) Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics 149(1):73-85
3) Mueller CL and Jaehning JA  (2002) Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex. Mol Cell Biol 22(7):1971-80
4) Krogan NJ, et al.  (2002) RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol Cell Biol 22(20):6979-92
5) Warner MH, et al.  (2007) Rtf1 is a multifunctional component of the paf1 complex that regulates gene expression by directing cotranscriptional histone modification. Mol Cell Biol 27(17):6103-15
6) Tous C, et al.  (2011) A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J 30(10):1953-64